摘要
采用尼龙毛柱法从脾淋巴细胞分离T、B淋巴细胞。通过MTT法检测,WPS-1对T淋巴细胞具有明显的增殖活性,并表现出明显的剂量-效果关系,增殖的最佳浓度为40μg/mL,并且WPS-1比APS-2作用效果更好。采用半定量RT-PCR进一步研究WPS-1对T淋巴细胞分泌的细胞因子白细胞介素2(IL-2)、IL-4、IL-6、干扰素γ(IFN-γ)mRNA表达的影响。首先研究了各细胞因子mRNA随WPS-1作用时间的变化,WPS-1促进IL-2、IL-4、IL-6mRNA表达最佳作用时间都为24h,而促进IFN-γmRNA表达的最佳作用时间为12h。采用各细胞因子mRNA表达的最佳作用时间,进一步考察WPS-1浓度的影响,得到其对促进IL-2、IL-6、IFN-γmRNA表达的最佳浓度都为40μg/mL,而对IL-4则为20μg/mL。WPS-1可能对淋巴细胞的免疫功能具有调节作用。
A nylon wool column was used to separate of spleen T and B lymphocytes.Through the MTT assay,WPS-1 was found to significantly enhance proliferation of T lymphocytes.The optimal concentration of WPS-1 to promote proliferation was 40μg/mL.Semi-quantitative RT-PCR was performed to determine changes in cytokine mRNA expression.The influence of WPS-1 to cytokine interleukin-2（IL-2）,IL-4,IL-6,and interferon γ（IFN-γ）mRNA expression in T lymphocytes were investigated.First,the relation between time of T lymphocytes cells incubated with WPS-1 and the cytokine mRNA expression was studied.The best time of WPS-1 to promote IL-2,IL-4,IL-6 mRNA of expression were all 24h.The best time to that of IFN-γ was 12h.The best time of the cytokines mRNA expression were adopted,to further investigate the effect of the concentration of WPS-1 to cytokine mRNA expression.The results showed the best concentration of WPS-1 to promote IL-2,IL-6,and IFN-γ mRNA expression were all 40μg/mL,to that of IL-4 was 20μg/mL.The results indicated that rapeseed polysaccharide could enhance the immune regulation of spleen T lymphocyte.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第8期380-383,488,共5页
Science and Technology of Food Industry
基金
国家"十五"重大科技专项资助项目(200lBA501A20)
湖北省重大科技攻关项目(2006AA20lB32)
关键词
菜籽多糖
脾淋巴细胞
免疫作用
rapeseed polysaccharide
spleen lymphocyte
immunomodulating effects
