摘要
目的:在前期的研究基础上,通过化学分析和药理活性分析相结合的方法,制备小续命汤抗脑缺血有效成分组。方法:DPPH法测定样品对自由基的清除作用;MTT法测定细胞存活率,观察样品对过氧化氢损伤、谷氨酸损伤及低氧损伤的保护作用;TBA法测定线粒体脂质过氧化。结果:综合分析多靶点活性,40%乙醇洗脱物和中间层的活性最强。结论:40%乙醇洗脱物和中间层按比例混合为小续命汤抗脑缺血有效成分组。
Objective： On basis of preliminary studies, to prepare the effeetive^components group of Xiaoxunfing decoction for anti-ischemie by combining chemical analysis with pharmacological activity screening. Method： Free radical scavenging was assayed by DPPH method; the cell viability injured by hydrogen peroxide, L-glutamate, and hypoxia was determined by MTT assay ; TBA meth- od was used to determine mitochondrial lipid peroxidation. Result： Comprehensive analysis of multi-target results, the comprehensive activities of 40% ethanol elution and the middle layer were the highest. Conclusion： 40% ethanol elution and the middle layer were proportionally mixed as the effective components group of Xiaoxuming decoction.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第15期2140-2144,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金重点项目(30630073)
关键词
小续命汤
有效成分组
脑缺血
Xiaoxuming decoction
effective components group
cerebral ischemic
