摘要
目的应用高通量筛选技术方法,建立新型的中药复方活性成分和作用机理研究模式,为中药现代化研究提供新思路和新技术。方法研究中药复方“小续命汤”240个连续组分(L1L120,A1A120)对β淀粉样蛋白毒性、过氧化损伤、谷氨酸损伤的保护作用及对β分泌酶活性的抑制作用。结果综合评价筛选结果,发现有3部分连续组分(L140,A3060,A100120)综合效果较好,这3部分重新组合作为此复方抗AD的有效成分组。结论中药复方“小续命汤”可以通过多组分、多靶点发挥抗AD作用。高通量筛选方法将极大地推进对中药复方的研究。
AIM： To apply high throughput screening methods to research Xiaoxuming Decoction＇s effective components combination and its action mechanism. METHODS： We studied the effects of 240 sequential components of Xiaoxuming Decoction （ L1-L120, A1-A120 ） on anti-Aβ neurotoxicity, anti-H2O2 damage, anti-glutamic acid damage and β-secretase activity. RESULTS： We evaluated these results of screening comprehensively, and found several components of Xiaoxuming Decoction （L1-IA0, A30-A60, A100-AI20） had the potential effects of listed before. So the combination of these components was regarded as the effective components combination of Xi- aoxuming Decoction for anti-Alzheimer＇s disease. CONCLUSION： Xiaoxuming Decoction had effective protection against Alzheimer＇s disease through multi-component and multi-target. High throughput screening methods will push the development of Chinese traditional compound prescription greatly.
出处
《中成药》
CAS
CSCD
北大核心
2005年第9期993-996,共4页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目:中药复方成分高通量筛选研究(No.30171148)
关键词
小续命汤
有效成分组
高通量筛选
阿尔茨海默病(AD)
Xiaoxuming Decoction
group of effective components
high-throughput screening
Alzheimer＇s disease
