摘要
目的:以4-甲基-7-羟基香豆素(Ⅰ)为原料通过Williamson醚法合成4-甲基-7-烯丙基氧香豆素(Ⅱ),对其进行生物转化研究,进一步探讨转基因何首乌毛状根体内酶系统的多样性。方法:Ⅰ在碳酸钾、碘化钾催化剂作用下与3-溴丙烯反应生成烯丙氧基产物Ⅱ,核磁共振技术进行结构鉴定;将Ⅱ投入何首乌毛状根悬浮培养体系中,共培养7 d后,利用TLC和HPLC进行产物检测,硅胶柱层析法分离纯化,核磁共振技术进行结构确认。结果:通过Williamson醚合成法成功得到目标产物Ⅱ。Ⅱ在何首乌毛状根悬浮培养体系中发生了生物转化反应。分离纯化得到两个化合物:4-甲基-7-羟基香豆素(Ⅰ)及4-甲基香豆素-7-O-β-D-吡喃葡萄糖苷(Ⅲ)。结论:何首乌毛状根酶系统具有水解酶及糖基转移酶活性,是良好的生物转化体系。
Objective：To synthesis 4-methyl-7-allyloxy coumarin by Williamson etherification from 4-methyl-7-hydroxy coumarin and apply as a substrate in hairy roots of Polygonum multiflorum.Methods：The synthesis reaction of 4-methyl-7-allyloxy coumarin used the allyl bromide and potassium carbonate as catalysts,and acetone as solvent reacted for 17 hours,then the product was isolated.4-methyl-7-allyloxy coumarin was added into the media of supension transgenic hairy roots of Polygonum multiflorum which had been pre-cultured for 8 d,and then co-cultured for another 7 d.The biotransformation products were detected by TLC and HPLC and isolated by various chromatographic methods.Results：Two biotransformation products,4-methyl-7-hydroxy coumarin and 4-methyl-coumarin-7-O-β-D-glucoside were isolated and identified.Conclusion：Hairy roots of Polygonum multiflorum contains not only glycosyltransferase but also hydrolysis enzymes.
出处
《中药材》
CAS
CSCD
北大核心
2011年第6期901-904,共4页
Journal of Chinese Medicinal Materials
基金
广东省自然科学基金(9451022401003453)
