摘要
目的:考察2,3-二氢环戊二烯香豆素类化合物对何首乌毛状根生长的影响,并研究其对毛状根中有效成分二苯乙烯苷累积的刺激作用。方法:将2,3-二氢环戊二烯香豆素类化合物在不同的培养时间加入到毛状根中,共培养特定时间后测定其二苯乙烯苷的含量及生长量,并通过考察不同浓度和继代次数确定最佳实验条件。结果:加入香豆素类化合物的最佳时间为预培养第4天,最佳质量浓度为0.025 g.L-1,毛状根的生长量有较大提高,与对照组相比二苯乙烯苷的含量增加4倍左右。结论:2,3-二氢环戊二烯香豆素类化合物作为一类非生物诱导子能够促进何首乌毛状根的生长及有效成分二苯乙烯苷的累积。
Objective： To investigate the influence of 2,3-dihydrocyclopenta coumarins to the growth of transgenic hairy root of Polygonum multiflorum and the accumulation of active ingredient stilbene glucoside in the culture.Method： In different culture time,2,3-dihydrocyclopenta coumarin compounds were administrated to the hairy roots.The yield of stilbene glucoside and the growth of the hairy roots were determined after some period of co-culture.Through the investigation of the different concentrations of the 2,3-dihydrocyclopentacoumarin,and sub-culture times,the optimal experimental conditions were established.Result： The optimal administration time of the 2,3-dihydrocyclopentacoumarin was on the fourth day,the optimal concentration of 2,3-dihydrocyclopentacoumarin was 0.025 g·L^-1.Under this condition,the growth of the hairy root greatly increased.Comparing with control group,the accumulation of stilbene glucoside was increased about four times.Conclusion： 2,3-dihydrocyclopentacoumarins could promote the hairy hair root growth and stimulate the stilbene glucoside accumulation.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第9期1197-1201,共5页
China Journal of Chinese Materia Medica
基金
广东省自然科学基金项目(04010461)
关键词
2
3-二氢环戊二烯香豆素
诱导作用
何首乌毛状根
二苯乙烯苷
2
3-dihydrocyclopentacoumarin
eliciting effects
transgenic hairy root of Polygonum multiflorum
stilbene glucoside
