摘要
目的:研究原癌基因人表皮生长因子受体2(HER-2)、细胞增殖标志物增殖细胞核抗原(PCNA)和凋亡相关基因Bcl-2、Bax在乳腺癌组织中的表达及其与淋巴结转移、临床分期等因素的关系。方法:应用免疫组化Envision二步法检测50例乳腺癌根治术后标本中HER-2、PCNA、Bcl-2、Bax蛋白的表达。结果:乳腺癌组织中HER-2、PCNA、Bcl-2、Bax的表达率分别为44%、82%、48%、54%。HER-2表达率在淋巴结转移组增高(P<0.05);PCNA表达率在肿瘤直径大组明显增高(P<0.01);Bcl-2表达率与年龄、肿瘤大小、淋巴结转移及临床分期无关;Bax表达率在淋巴结转移组及临床分期晚组增高(P<0.05)。相关分析显示,HER-2、Bax表达与淋巴结转移呈正相关(P<0.05)。结论:联合检测HER-2、PCNA、Bcl-2、Bax可作为判断乳腺癌预后的可靠指标。
OBJECTIVE:To study the expressions of HER-2,PCNA,Bcl-2 and Bax in breast cancer tissue and their relationships with lymph node metastasis,clinical stage and other prognostic factors.METHODS:The expressions of HER-2,PCNA,Bcl-2,and Bax in 50 specimens of breast cancer were detected by immunohistochemistry with Envision staining.RESULTS:The expression rates of HER-2,PCNA,Bcl-2 and Bax in breast cancer tissues were 44%,82%,48% and 54%,respectively.HER-2 positive expression rate was significantly high in lymph node metastasis cases (P0.05).PCNA expression was related with tumor size (P0.01).Bcl-2 expression had no significant relation with the age,tumor size,lymph node metastasis or clinical stage.Bax positive expression rate was significantly high in lymph node metastasis and late stage cases (P0.05).Correlative analysis indicated the expressions of HER-2 and Bax were up regulated with lymph node metastasis (P0.05).CONCLUSION:HER-2,PCNA,Bcl-2,and Bax can be markers for estimating the prognosis of breast cancer.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第30期2853-2855,共3页
China Pharmacy