摘要
目的探讨p53基因和bcl-2／bax基因在乳腺癌发病机制中的作用以及它们之间的相互关系。方法以小鼠乳腺癌BCML-TA299可移植模型为研究对象，用免疫组织化学染色法观测α-干扰素（INF-α）干扰后p53基因与bcl-2／bax表达的关系。结果实验性乳腺癌BCML-TA299治疗组荷瘤鼠生存期较对照组延长，且随着时间的推延治疗组肿瘤的体积明显低于对照组的肿瘤体积；治疗组与对照组比较，肺转移率低。对照组p53蛋白阳性表达率为92．01±0．48，而治疗组中p53呈阴性表达。对照组瘤组织bcl-2蛋白表达为50．21±0．52，bax蛋白表达为12．08±0．13；治疗组瘤组织bcl-2蛋白表达为12．01±0．2，bax蛋白表达为48．19±0．57。对照组p53蛋白表达与bcl-2呈正相关，而与bax呈负相关（P〈0．05）；治疗组中p53呈阴性表达，bcl-2和bax表达与对照组结果相反。结论bcl-2和bax的表达受p53调节。p53、bcl-2／bax在乳腺癌发病机制中起着较重要的作用。
Objective To observe the action and relationship between p53 and bcl-2/bax in breast cancer. Methods Taking rat breast cancer BCML-TA299 model as research subjects, the relationship between p53 and bcl-2/bax following IFNα interference was observed by using immunohistochemistry. Results Rats in interferon group survived longer than those in control group. Compared to control group, rats in interferon group had smaller size of tumor and lower percent of lung metastasis. There was positive expression of p53 in inerferon group but negative expression in control group. The expression of bcl-2 was 50.21 ±0.52 and bax was 12.08 ±0.13 in control group; The expression of bcl-2 was 12.01 ± 0.2 and bax was 48.19 ± 0.57 in interferon group. The expression of p53 was positively correlated with bcl-2 and negatively with bax. Conclusion The expression of bcl-2 and bax are regulated by the expression of p53. p53, bcl-2 and bax play important roles in the pathogenesis of bresat cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第2期176-178,共3页
Chinese Journal of Experimental Surgery
