摘要
目的:研究MAV S与p21的相互作用,对MAVS通路的调节。方法:我们首先通过PCR的方法克隆了全长的p21基因,然后通过co-IP方法进一步验证p21与MAVS是否可以发生相互作用,接下来通过荧光素酶报道基因的方法研究p21对MAVS通路的影响。结果:通过co-IP发现p21与MAVS可以发生相互作用,另外p21降低MAVS活性。结论:成功地克隆了p21基因,p21与MAVS在293T细胞内可以发生相互作用,p21可以抑制MAVS激活NF-κB途径和IFN-β途径的活性,为进一步研究p21在MAVS通路中所起的作用奠定基础。
AIM： To study the interaction between MAVS and p21 regulates MAVS-mediated pathway.METHODS： Firstly,we cloned full-length p21 gene by PCR.And then the interaction between MAVS and p21 was further demonstrated by co-immunoprecipitation.The regulation of MAVS-mediated pathway by p21 was analyzed by luciferase reporter assays.RESULTS： There are interaction between p21 and MAVS in 293T cells.p21 downregulates activity of MAVS.CONCLUSION： It is shown that p21 inhibits MAVS-mediated activation of NF-κB and IFN-β by Luciferase reporter assays.Overall,these results laid the foundation for further investigating the function of p21 in MAVS-mediated pathway.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2011年第8期832-834,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(8100076381071321)
