摘要
目的:通过研究晚期糖基化终产物受体(RAGE)、核转录因子NF-κB、p21在幼、老年C57BL/6j小鼠螺旋神经节细胞(SGC)的表达,探讨老年性聋的发病机制。方法:将C57BL/6j小鼠分为2月龄、10月龄组各25只,分别行组织学切片观察耳蜗SGC形态结构,免疫组织化学检测RAGE、NF-κB、p21在SGC中的表达,利用IPP6图像分析软件计算平均光密度值,值越大,表达量越多。结果:①10月龄小鼠SGC数目明显比2月龄少,分别为(20±6)个和(39±5)个,差异有统计学意义(P<0.01);②RAGE在2月龄和10月龄小鼠耳蜗SGC平均光密度值分别为0.179±0.025和0.308±0.050;NF-κB在2月龄和10月龄小鼠耳蜗SGC平均光密度值分别为0.181±0.045和0.335±0.120;p21在2月龄和10月龄小鼠耳蜗SGC平均光密度值分别为0.160±0.023和0.365±0.031,两组分别比较,差异均有统计学意义(P<0.05)。结论:RAGE、NF-κB、p21在SGC中有表达且随年龄增大表达增多,推测RAGE、NF-κB、p21可能参与老年性聋的发病过程。
Objective：To research advanced glycation end-product receptors（RAGE）, NF-kB,p21 expressions in C57BL/6] mice cochlea spiral ganglion cells（SGC） ,and then to investigate the presbyacusa pathogenesis. Meth-od：To take C57BL/6J mice： 2 month 25,and 10 month 25. Histological sections were observed the SGC. RAGE, NF-kB, p21 were immunohistochemical in the SGC,with IPP6 to IOD. Result： O The count SGCs of 10 month-old was obviously decreased comparing to 2 month-old, the count of 2 month SGC is 39± 5,10 month group is 20±6, P〈0.01 ;（2）RAGE, NF-kB ,p21 expressed in spiral ganglion cell,different place with different age,and the means optical density in the 10 month are higher than the 2 month, respectively. The IOD of RAGE expression in 2 month SGC：0. 179±0. 025,10 month IOD：0. 308±0. 050;The TOD of NF-kB expression in 2 month SGC：0. 181± 0. 045,10 month IOD：0. 335±0. 120;The IOD of p21 expression in 2 month SGC：0. 160±0. 023,10 month IOD： 0. 365±0. 031, compare with 2 group, respectively＇, P〈O. 05, and the difference has statistics sense. Conclusion： RAGE,NF-kB,p21 expressions are in SGCs and increases with the aging of SGCs, suppose RAGE, NF-kB, p21 may participate in the process of preshyacusa pathogenesis.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2014年第4期265-268,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
武汉市卫计委临床医学科研项目(No:WX13B09)
