摘要
采用硅胶柱层析、JTY树脂分离制备人参皂苷Rb3,薄层板(TLC)跟踪,高效液相色谱法(HPLC)检测。目的是研究西洋参叶中不同浓度的人参皂苷Rb3对胰脂肪酶的抑制作用影响,从而表明人参皂苷Rb3在抗肥胖中的作用。用制得的人参皂苷Rb3,在卵磷脂所乳化的甘油三酯的检测系统中进行体外胰脂肪酶抑制实验。结果表明:制得的人参皂苷Rb3经过三种不同展开剂,薄层板展开,均为一个点,进一步经HPLC检测其纯度为93.2%。胰脂肪酶抑制试验结果表明人参皂苷Rb3在浓度为0～1 mg/mL时,其抑制率达到85.11±0.409%,而且远远高于西洋参叶提取物。本文通过胰脂肪酶抑制试验表明Rb3具有抗肥胖作用,其抑制机制有待进一步研究。
Ginsenoside Rb3 of American ginseng leaves was isolated by the methods of silica column chromatographic and JTY resin,tested by thin-layer chromatography（TLC）,and examined by high performance liquid chromatography （HPLC）.The experiments studied the effects of different concentration ginsenoside Rb3 isolated from American ginseng leaves on pancreatic lipase activity,which could reflect its anti-obesity function.The effect of Ginsenoside Rb3 on porcine pancreatic lipase activity was determined by measuring released free fatty acids after incubating in substrate emulsions containing bile salts and phosphatidylcholine in vitro.Ginsenoside Rb3 inhibited the pancreatic lipase activity in dose-dependent manner at the concentrations of 0.25-1 mg/mL.It inhibited hydrolysis of about 85.11±0.409% of trioein at about 1 mg/mL of ginsenoside Rb3,which was much more higher than the American ginseng leaves extract.The article manifested that ginsenoside Rb3 has the anti-obesity function through the pancreatic lipase experiment,which inhibitory mechanism pending further studies.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第3期522-525,共4页
Natural Product Research and Development
