摘要
以柚皮为原料,用乙醇提取柚皮中的总黄酮,对其抗氧化活性进行研究。通过单因素试验和正交试验确定最佳提取条件,试验表明:乙醇浓度80%、料液比1∶20(g/mL)、提取温度85℃,提取时间2 h,柚皮黄酮得率最高,达到1.35%。抗氧化实验表明,柚皮黄酮提取物具有较强的清除DPPH.和.OH自由基的能力,其EC5(0半抑制浓度)分别为0.036 mg/mL和0.495 mg/mL。
The extraction and antioxidant activity of flavonoids from pomelo peel was investigated.The optimal combinations for the extraction conditions were ethanol concentration 80%,ratio of solid to liquid 1∶20（g/mL）,extracting temperature 85 ℃,and extracting time 2 h,by which the extraction quality of flavonoids from pomelo peel was 1.35 %.Moreover,antioxidation experiments showed that flavonoids from pomelo peel have strong scavenging capabilities for DPPH· and ·OH free radical,their EC50 values were 0.036 mg/mL and 0.495 mg/mL,respectively.
出处
《食品研究与开发》
CAS
北大核心
2011年第6期25-28,共4页
Food Research and Development
基金
仲恺农业工程学院校级科研项目(G3091417)
关键词
柚皮
总黄酮
提取
抗氧化活性
pomelo peel
total flavonoids
extraction
antioxidant activity
