摘要
目的探讨海带对四氧嘧啶糖尿病（DM）大鼠血糖影响的机制。方法成年健康雌性Wister大鼠60只，采用随机数字表法随机取10只作为正常对照组，其余50只腹腔注射四氧嘧啶建立高血糖大鼠模型。造模成功的40只按随机数字表法分为DM模型组、治疗A组（低剂量海带饲料，相当于2．5g／kg·d^-1体重）、治疗B组（中剂量海带粉饲料，相当于10g／kg·d^-1体重），治疗C组（高剂量海带粉饲料，相当于25g/kg·d^-1体重），每组各10只。检测大鼠空腹血糖（FBG）、血清胰岛素（Insulin）水平，观察胰岛细胞的形态结构，检测胰岛细胞超氧化物歧化酶（SOD）和诱导性一氧化氮合酶（iNOS）的表达。结果治疗B组、C组大鼠血清FBG水平[分别为（9．37±1．70）mmol／L、（9．18±1．65）mmol／L]较DM模型组[（12．63±1．67）mmol／L]显著降低（F=32．81，q=6．35～11．72，P〈0．05），治疗A组、B组、c组血清Insulin水平吸光度值A[分别为（0．0378±0．0026）、（0．0378±0．0027）、（0．0367±0．0035）]均较DM模型组（0．0456±0．0057）升高（F=11．40，q=4．28～8．47，P〈0．05），胰岛细胞组织结构病变减轻，治疗B组、C组大鼠胰岛细胞SOD表达较DM模型组明显增强（f=4．73～4．76，P〈0．05），iNOS表达较DM模型组显著降低（t=4．81～5．30，P〈0．05）。结论海带可通过增强抗氧化作用，促进胰岛细胞分泌功能恢复而降低血糖。
Objective To investigate the hypoglycemic effects ofLaminariajaponica （L. japonica） on diabetic model induced by alloxan in rats. Methods Sixty healthy female rats were used to establish diabetic models by injecting alloxan peritoneally, and L.japonica was applied as raw materials for potential marine drugs. The levels of fasting blood glucose （FBG） were detected by automatic blood glucose device. Enzyme linked immunoabsorbant assay was applied to determine the insulin level in serum. The shape and structure of islet cells were observed with histopathologieal staining, and the expression of superoxide dismutase （SOD） and inducible nitric oxide synthase （iNOS） in islet cells were detected by immunohistoehemical technique. Results After the treatment, the levels ofFBG of L. japonica treated group B [（9.37± 1.70） retool/Land C （9.18± 1.65 ） mmol/L, F= 32.81, q = 6.35± 11.72, P〈 0.05 ] reduced, while the serum levels of insulin in treated group A, B and C （0.0378± 0.0026, 0.0378±0.0027, 0.0367± 0.0035） increased （F= 11.40, q =4.28-8.47, P〈 0.05） significantly than those of diabetic model group （0.0456± 0.0057） . The shape and structure of islet cells improved with the up-expressing SOD （t=4.73-4.76, P〈0.05）and down-expressing iNOS （t=4.81-5.30, P〈 0.05） in L.japonica treated group B and C than those in diabetic model group. Conclusion L.japoniea might decrease the serum level of FBG through promoting the islet cell recovery by an anti-oxide effect.
出处
《国际中医中药杂志》
2011年第7期597-600,共4页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目（项目编号：40976085）,威海市科技局项目（项目编号：200913,2009-3-74）
关键词
海带
糖尿病
四氧嘧啶
氧化应激
Laminariajaponica
Diabetes mellitus
Alloxan
Oxidative stress
