摘要
以Prins×PI170914／7*PrinsF3分离群体对在Prins与其Pm6近等基因系P1170914／7*Prins之间呈现多态性的RFLP标记进行遗传作图，发现8个多态性标记中有3个（Xbcd135EcORV9kb、Xbcd307EcOR18．8kb、Xbcd266EcORV18kb）与转移到普通小麦2B染色体长臂上的来源于T．timopheevi的Pm6基因位点紧密连锁。用这8个多态性标记对国际公认含Pm6基因的小麦品种和品系进行检测时表明，这些标记中Xbcd135-2BL可有效地跟踪已导入不同小麦遗传背景中的Pm6基因。
The Pm6 gene from T timopheevi confers resistance to wheat Erysiphe graminis f.sp. tritici. A total of 8 RFLP markers were selected for their ability to reveal polymorphism on powdery mildew resistant ‘Prins’ near-isogenic lines (with Pm6) and the recurrent parent Prins. The linkage relationship betWeen the polymorphic markers and Pm6 gene was estimated with a F2 population from cross of NIL with Prins. It was indicated that three markers Xbcd135EcoRV9kb, Xbcd307EcoR18.8kb and Xbcd266EcoRV18kb are closely linked to the gene locus with distances 1.6±1.5cM, 1.6±1.5cM, 4.8±2.6cM, respechvely. Eigteen wheat culhvars or lines reposed as containing Pm6 genes were employed to assess the applicability of the three markers for idenhfication of An6 gene in different genetic background. Results showed that marker Xbcd135-2BL can be used to tag the presence of Pm6 gene introgressed to different wheat genetic background.
基金
国家863计划及自然科学基金
关键词
小麦
白粉病抗性
Pm6近等基因系
RFLP标记
T. aestivum, Powdery mildew resistance, Near-isogenic line, Pm6 locus,RFLP marker
