摘要
目的 探讨甲状腺乳头状癌（PTC）组织中Runt相关转录因子3（Runx3）基因mRNA和蛋白表达及意义.方法 采用反转录一聚合酶链反应（RT-PCR）和Western印迹技术,检测了67例PTC及其对应癌旁组织中Runx3基因mRNA及蛋白的表达.结果 Runx3 mRNA在PTC中的表达量相对值（0.31 ±0.07）低于癌旁甲状腺组织的表达量（0.92 ±0.08）（P〈0.05）.Runx3基因mRNA的表达与患者的病理分级及淋巴结转移有关（P〈0.05）.Runx3蛋白在PTC中的积分吸光度（A）值为（1012±221）,明显低于癌旁组织的积分吸光度（A）值（1993±199）（P〈0.05）.Runx3蛋白表达与患者的TNM分期、病理分级及淋巴结转移有关（P〈0.05）.Runx3基因mRNA的表达与Runx3蛋白的表达有相关性（P〈0.05）.结论 Runx3 mRNA及其蛋白在PTC表达均低于癌旁组织,Runx3基凶的表达异常在PTC的发生、发展中起着重要的作用.
ObjectJve To explore the mRNA and protein expressions of Runx3 gene in papillary thyroid carcinoma（PTC）.Methods Reverse transcription-polymerase chain reaction（RT-PCR）and Western blot were used to detect the Runx3 mRNA and protein levels in 67 human PTC specimens and their matched adjacent non-cancerous epithelium（NCE）.Results The relative expression value of Runx3mRNA was 0.31 ±0.07 in PTC vs 0.92 ±0.08 in NCE（t=38.251,P〈0.05）.In PTC.it was correlated with the tumor pathological grade and lymph node metastasis（x^2=5.511,6.492,P〈0.05）.The integral optical density value of Runx3 protein confirmed was 1012±221 in PTC vs 1993 ±199 in NCE（t=18.413.P〈0.05）. In PTC,it was correlated with the tumor TNM stage.pathological grade and lymph node metastasis（X2=5.550,9.678,5.070,P〈0.05）.Furthermore there was a distinct correlation between the mRNA and protein expressions of Runx3 gene（X2=42.699,P〈0.05）. Conclusions The mRNA and protein expressions of Runx3 gene in PTC were lower than those in NCE.A lower expression of Runx3 gene may play an important role in the carcinogenesis and progression of PTC.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2011年第20期1393-1396,共4页
National Medical Journal of China
基金
河南省医学科技攻关项目（201003036）
关键词
甲状腺肿瘤
RUNT相关转录因子3
RT—PCR
免疫印迹法
Thyroid neoplasms
Transcription factor 3
Reverse transcription-polymerase chainreaction
Western blot
