摘要
目的观察血清MG7-Ags、IL-6R、VEGF在中晚期胃癌患者介入干预前后水平变化。方法选择中晚期胃癌患者50例,其中介入干预30例,静脉化疗20例,化疗药物选用5-氟脲嘧啶、亚叶酸钙及草酸铂,分别在干预前、干预后2周、干预后4周,清晨空腹留取静脉血5 ml,室温下静置半小时,再3 000 r/min离心10 min,留取血清进行检测,MG7-Ags、IL-6R、VEGF的测定采用ELISA法。结果介入干预组中MG7-Ags、IL-6R的水平呈下降趋势,在干预后4周、干预后2周与干预前比较均有显著性差异,干预后4周与干预后2周比较亦有显著性差异,VEGF的水平在干预后2周较干预前有所增高,但干预后4周却是下降的,比较差异有显著性;静脉化疗组中MG7-Ag、VEGFs、IL-6呈下降趋势,干预后4周与干预前比较均有显著性差异,其中只有sIL-6R干预后4周与干预后2周比较有显著性差异,而MG7-Ag、VEGF干预后4周与干预后2周无显著性差异;介入干预组中MG7-Ag、VEGFs、IL-6R的水平变化较静脉化疗组明显,两组组间比较均有显著性差异(分别为F=4.356,P=0.042<0.05、F=4.078,P=0.049<0.05,F=4.389,P=0.041<0.05)。结论通过对中晚期胃癌患者动脉介入干预前后血清MG7-Ags、IL-6R、VEGF动态观察,上述三项指标在干预后均有变化,但介入干预组中MG7-Ag、VEGFs、IL-6R的水平变化较静脉化疗组明显,故MG7-Ags、IL-6R、VEGF可作为评估胃癌干预疗效及预后的一个重要参考指标。
Objective To investigate the levels of MG7-Ag、sIL-6R、VEGF in serum before and after transcatheter arterial chemo-embolization in patients with advanced gastric.Methods 30 patients who were treated with interventional treatment and 20 patients who were treated with intravenous chemotherapy.Fasting venous blood（5 ml） were collected from 50 cases with clinical in the morning,respectively on the 1st day before the treatment,the 1st,two weeks and the 1st,four weeks after treatment.The serm were static for 30 minutes,rotated for 10 minutes with 3 000 r/min.At low temperature,the serum was separated and stored in-70℃,MG7-Ag、sIL-6R、VEGF was detected with ELISA,observed the changes of MG7-Ag、sIL-6R、VEGF.Results Serum MG7-Ag、sIL-6R levels at the 1st day before the TACE,at the 1st day,two weeks,and four weeks after the treatment was significantly decreased.At the 1st day,two weeks and 1st day,four weeks operation when compared with 1st before operation,there was statistically significant difference（P0.05）.It significantly decreased 1st four weeks compared with 1st two weeks after operation,there was statistically significant difference（P0.05）.Serum MG7-Ag、sIL-6R levels decreased after interventional treatment,it significantly decreased four weeks compared with 1st day before interventional treatment.There was statistically significant difference（P0.05）,sIL-6R significantly decreased 1st four weeks compared with 1st two weeks after the treatment,however MG7-Ag、VEGF there was not statistically significant difference 1st four weeks compared with 1st two weeks after the treatment.The level of Serum MG7-Ag、sIL-6R changed more obviously after the interventional treatment compared with the intravenous chemotherapy,there was statistically significant difference.（F=4.356,P=0.0420.05、F=4.078,P=0.0490.05,F=4.389,P=0.0410.05）.Conclusion To observe changes in serum MG7-Ag、sIL-6R、VEGF before and after transcatheter arterial chemo-embolization in patients with advanced gastric cancer.The lev
出处
《中国实验诊断学》
北大核心
2011年第5期853-856,共4页
Chinese Journal of Laboratory Diagnosis
