摘要
目的研究新的血管生成抑制剂2(8羟基6甲氧基1氧1氢2苯并吡喃3烃基)丙酸(NM3)对胃癌生长的抑制作用,并探讨其对胃癌细胞凋亡的影响。方法建立人胃腺癌裸鼠皮下异体移植模型。移植1周后开始腹腔注射NM3,隔天1次,剂量为10、20、40mg/kg,分别3d1次联合卡铂5mg/kg,并与生理盐水对照组、NM3或卡铂单独治疗组比较(NM3用5周,卡铂4周)。种植后第7周处死动物,测量肿瘤大小,计算抑瘤率,流式细胞术(FCM)法分析肿瘤细胞凋亡指数(AI)。结果单用NM3剂量分别为10、20及40mg/kg时,肿瘤的平均重量分别为(1351.0±116.9)、(1041.1±143.5)及(767.5±140.1)mg,明显小于生理盐水对照组[(1754.0±144.2)mg,P<0.05]。单用卡铂组的抑瘤率为5.6%,对胃癌无明显抑制作用。单用NM3剂量为10、20及40mg/kg时,抑瘤率分别为23.0%、40.6%及56.2%。三组剂量分别联合卡铂治疗时抑瘤率为42.9%、47.6%及63.2%,与对照组比较差异有统计学意义(P<0.05)。NM3三种剂量组联合卡铂治疗的AI分别是(6.96±0.65)%、(10.65±1.43)%及(21.66±2.96)%,对照组为(1.37±0.19)%,卡铂组为(1.85±0.22)%。结论NM3能诱导胃癌细胞凋亡,对体内胃癌的生长有抑制作用,能加强卡铂对胃癌的化疗疗效。
Objective To study the effect of an novel angiogenesis inhibitor, NM-3, on primary tumor growth of human gastric cancer in vivo, and to examine apoptosls of gastric cancer cells induced by NM-3. Methods We established xenotransplanted model of a human gastric cancer cell SGC-7901 in the dorsal skin of nude mice. NM-3 was injected peritoneally at dose of 10 mg/kg, 20 mg/kg or 40 mg/kg every other day for 5 weeks, combined with carboplatin （5 mg/kg） every third day for 4 weeks. As controis of combined treatment, the another two groups of mice were injected with NM-3 or carboplatin alone. Seven weeks after implantation, tumor size, tumor growth inhibition rate, and apoptotic index （AI） were evaluated respectively after sacrifice of the mice. Results Compared with the untreated con- trois, growth of the tumor implanted subcutaneously was significantly reduced in size in the mice treated with NM-3 10 mg/kg, 20 mg/kg, or 40 mg/kg , with an inhibition rate of 23.0% ,40.6% and 56.2%, respectively. The AI increased significantly in the mice treated with NM-3 combined with carboplatin （ 1.37 % ± 0.19 % versus 6.96 % ± 0.65 % , 10.65 % ± 1.43 % , and 21.66 % ± 2.96 % ）. Conclusions NM-3, as an angiogenesis inhibitor, can induce apoptosis in gastric cancer and has strong inhibitory effect on tumor growth of human gastric cancer implanted in nude mice.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2005年第7期406-408,共3页
Chinese Journal of Digestion
