摘要
目的研究肝细胞生长因子(HGF)受体即c-Met在大肠癌组织中的表达及其与微血管密度(MVD)的关系,探讨c-Met在大肠癌发生、发展中的作用。方法采用RT-PCR方法检测大肠癌组(80例)、大肠腺瘤组(60例)及大肠正常黏膜组(40例)3组组织中c-Met mRNA的表达,应用免疫组织化学法检测组织中c-Met蛋白和以CD105为标记的MVD的表达,评价c-Met和MVD表达与大肠癌临床病理指标的关系。结果 c-Met在71.3%(57/80)的大肠癌组织和31.7%(19/60)的大肠腺瘤组织和17.5%(7/40)大肠正常黏膜组织中呈阳性表达。大肠癌组织中c-Met在mRNA水平和蛋白水平均高于大肠腺瘤组织和大肠正常黏膜组织(P<0.01)。c-Met和MVD的表达与大肠癌的淋巴结转移、浸润深度、分化程度和Dukes分期相关(P<0.05),而与患者的性别、年龄、原发部位及肿瘤大小无关(P>0.05)。c-Met表达与微血管密度呈正相关(r=0.034 6,P<0.05),c-Met表达阳性的组织中MVD值高于c-Met表达阴性者(P<0.01)。大肠癌伴肝转移者c-Met和MVD的表达明显高于无肝转移者(P<0.01)。结论 c-Met在大肠癌的侵袭与转移中可能发挥着重要作用,联合检测c-Met和MVD的表达有助于大肠癌预后的判断。
Objective To investigate expression of c-Met,the protein tyrosine kinase receptor for hepatocyte growth factor/scatter（HGF） in colorectal cancer,its relationship with micro-vessel density（MVD）,and its role in progression and metastasis of colorectal cancer.Methods C-Met expression was detected at the protein level by immunohistochemistry and at the mRNA level by semi-quantitative reverse transcriptase-polymerase chain reaction in colorectal adenocarcinomas（80 cases）,colorectal adenoma（60 cases） and normal mucosa（40 cases）.MVD expression marked with CD 105 was determined and calculated by immunohistochemistry.Correlation of C-Met and MVD expressions and their association with clinicopathological features and outcome were analyzed.Results C-Met-positive rate was 71.3%（57/80） in colorectal cancer,31.7%（19/60） in colorectal adenoma and 17.5%（7/40） in normal mucosa.C-Met expression in colon cancer was significantly higher than that in colorectal adenoma and normal mucosa（P〈0.01）.C-Metand MVD expressions were associated with lymph node metastasis,tumor differentiation and Dukes stage（P〈0.05）.c-Met expression was correlated with MVD（r=0.034 6,P〈0.05）.MVD in the c-Met（＋） group was higher than that in the c-Met（-） group（P〈0.01）.Also,a significant increase in c-Met and MVD was detected in colorectal cancer with liver metastasis compared with primary colorectal cancer（P〈0.01）.Conclusions c-Met may play an important role in progression and metastasis of colorectal cancer.Moreover,combined evaluation of c-Met and MVD expressions might be a useful indicator for liver metastasis in patients with colorectal cancer.
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第5期90-93,共4页
Journal of Shandong University:Health Sciences
