摘要
目的:观察白藜芦醇(resveratrol,Res)对兔急性高眼压条件下视网膜节细胞(retinal ganglial cells,RGC)核因子-κB(nuclear factor-kappe B,NF-κB)表达及血清中白细胞介素-6(interleukin-6,IL-6)浓度的影响。方法:将新西兰大白兔随机分成处理组、对照组和正常组。处理组于急性高眼压模型建立前7d,每天给予Res30mg/kg(3mL)ip,第8d经前房灌注升高眼压达90mmHg(1kPa=7.5mmHg),持续90min,24h后取材;对照组用等剂量的生理盐水代替Res给予ip,余同处理组;正常组不做上述处理直接取材。观察兔视网膜组织病理学结果,免疫组织化学检测RGC中NF-κB表达及血清中IL-6浓度的变化。结果:与正常组相比,处理组和对照组表达在RGC的NF-κB明显增加,外周血清中IL-6浓度明显增高(P<0.05);与对照组相比,处理组NF-κB的表达明显减少,外周血清中IL-6浓度明显降低(P<0.05)。结论:Res通过降低兔急性高眼压RGC中NF-κB的表达和血清中IL-6浓度,减轻RGC的损伤,对视网膜具有一定的保护作用。
AIM：To observe the effect of resveratrol on expression of nuclear factor-kappe B （NF-κB） and IL-6 concentration in acute ocular hypertension rabbits model.METHODS：The New Zealand rabbits were randomly divided into treated group, control group and normal group. Before setting up the model of acute high intraocular pressure,the resveratrol 30mg/kg（3mL） was injected into abdominal cavity of treated group’s rabbits for 7 days, an acute ocular hypertension model of 90 mmHg （1kPa=7.5mmHg） was induced after 90 minutes’ anterior chamber infusion at the eighth day . Specimen was obtained at 24 hours after the ocular hypertension. In control group normal saline was used instead of resveratrol; For the normal group, specimen was obtained without the above treatment. The specimen was removed for microscopic examination, and the expression of NF-κB was studied by immunohistochemical examination, and blood serum was collected to detect IL-6 concentration.RESULTS：Compared with the normal group, the expression of NF-κB in retinal ganglial cells（RGC） and IL-6 concentration in treated group and control group were significantly up-regulated; Compared with the control group, the expression of NF-κB in RGC and IL-6 concentration in treated group were significantly decreased.CONCLUSION：Through decreasing the expression of NF-κB and IL-6 concentration, resveratrol can reduce the RGC damages. It may play a protective role on the retina in glaucoma pathology
出处
《国际眼科杂志》
CAS
2011年第6期958-960,共3页
International Eye Science
