摘要
目的研究Bcl-xL基因特异性RNA干扰对前列腺癌PC-3细胞株体外增殖能力和凋亡的影响。方法构建靶向Bcl-xL的小干扰RNA(siRNA)表达载体,转染PC-3细胞后,采用半定量RT-PCR和Western blot法检测转染前后Bcl-xL mRNA及蛋白表达水平的改变;采用噻唑兰(MTT)法检测细胞生长增殖情况;TUNEL试剂盒测定细胞的凋亡情况。结果靶向Bcl-xL的序列特异性的siRNA可以有效地抑制PC-3细胞Bcl-xL基因的表达。转染靶向Bcl-xL的siRNA表达质粒可以显著抑制PC-3细胞的增殖(P<0.001),诱导细胞凋亡(P<0.001)。结论 Bcl-xL基因特异性RNA干扰可以显著抑制前列腺癌PC-3细胞的体外增殖并诱导细胞凋亡。
Objective To investigate the effect of siRNA targeted against Bcl-xL on cell proliferation and apoptosis of PC-3 prostate cancer cells.Methods A siRNA plasmid expression vector against Bcl-xL was constructed and transfected into PC-3 cells.The changes of Bcl-xL mRNA and protein expression were detected by semi-quantitive RT-PCR and Western blot respectively.Cell proliferation was detected by MTT assay.Cell apoptosis was evaluated by flow cytometry.Results The sequence-specific siRNA effectively suppressed Bcl-xL expressing at both mRNA and protein levels.Bcl-xL expression significantly inhibited the proliferation（P0.001）and induced apoptosis of PC-3 cells（P0.001） respectively.Conclusion siRNA targeted against Bcl-xL could effectively suppress PC-3 cell proliferation and significantly induce PC-3 cell apoptosis.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2011年第5期509-511,共3页
Cancer Research on Prevention and Treatment
