摘要
目的:通过检测罗格列酮(ROS)作用前后人喉癌Hep-2细胞核因子(NF-κB)和血管内皮生长因子(VEGF)mRNA表达的变化情况,探讨其抑制Hep-2增殖的机制。方法:采用MTT法检测12.5、25.0、50.0和100.0μmol/L ROS处理12、24、36、48、60和72 h后Hep-2细胞的增殖抑制率。RT-PCR方法检测50μmol/L的ROS处理0、24、48和72 h后Hep-2细胞NF-κB和VEGF mRNA表达的变化。结果:ROS对Hep-2细胞的生长具有增殖抑制作用,其作用表现为剂量依赖性(12、24、36、48、60和72 h各浓度组相比,F为117.584、98.241、92.352、117.228、148.458和124.900,P均<0.001)和时间依赖性(12.5、25.0、50.0和100.0μmol/L各时间组相比,F为140.949、123.335、148.325和176.590,P均<0.001)。RT-PCR检测结果显示50μmol/L的ROS处理Hep-2细胞0、24、48和72 h后,NF-κB相对表达量分别为(0.854±0.066)、(0.653±0.059)、(0.489±0.027)和(0.336±0.031),差异有统计学意义(F=111.357,P<0.001);VEGF相对表达量分别为(0.756±0.057)、(0.628±0.039)、(0.482±0.044)和(0.318±0.035),差异有统计学意义(F=89.796,P<0.001)。结论:ROS具有抑制Hep-2细胞增殖的作用,其机制与下调NF-κB和VEGF mRNA表达有关。
Aim： By detecting nuclear factor-KB（NF-KB）and vascular endothelial growth factor（VEGF） expression changes in human laryngeal carcinoma Hep-2 cells before and after being treated with rosiglitazone （ROS） , to explore its role in Hep-2 proliferation inhibition. Methods：MTT method was used to observe the proliferation of Hep-2 cells treated with 12.5,25.0,50.0, and 100.0 i.μmol/L ROS for 12,24,36,48,60,and 72 hours. RT-PCR method was used to detect the expressions of NF-KB and VEGF mRNA in the Hep-2 cells treated with ROS at 50μmol/L for 0,24,48 ,and 72 hours. Results： ROS inhibited growth of Hep-2 cells in a dose-dependent （at 12, 24, 36, 48, 60, and 72 h each concentration group,F were 117. 584, 98. 241 , 92. 352, 117. 228, 148. 458, and 124. 900, P 〈 0. 001 ） and time-dependent manner （12.5, 25. 0,50. 0, and 100. 0 μmol/L groups at different time point, F were 140. 949, 123. 335, 148. 325, and 176. 590, P〈0.001）.The expression ofNF-s：B mRNA was （0.854 ±0.066）, （0.653 ±0.059）, （0.489±0.027）, and （0. 336 ± 0.031 ） at 0, 24, 48, and 72 h after the treatment by ROS at 50μmol/L, and there was significant differ- ence among different time groups（F = 111.357,P 〈0.001）.The expression of VEGF mRNA was （0.756 ±0.057）, （0.628±0.039）, （0.482 ±0.044）, and （0.318 ±0.035） at0, 24, 48, and 72 h,and there was significant difference among different time groups（ F = 89. 796,P 〈 0. 001 ）. Conclusion： ROS could inhibit Hep-2 cell proliferation,and its mechanism is related to down-regulating NF-KB and VEGF mRNA expression.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2010年第2期262-265,共4页
Journal of Zhengzhou University(Medical Sciences)
