摘要
将中空纤维液相微萃取(HFLPME)-高效液相色谱法(HPLC)与Bjerrum或Scatchard法结合,同时、快速研究了6种黄酮类化合物的蛋白结合率、结合常数和结合位点数。最佳萃取条件为:聚偏氟乙烯作为有机溶剂载体,正庚醇作为萃取相,搅拌速度900 r/min,萃取时间2 h。在最佳条件下,二氢杨梅素、杨梅素、槲皮素、山柰素、异鼠李素和白杨素与BSA结合率分别为29.3%,56.8%,12.2%,25.7%,25.2%和12.8%。6种黄酮类化合物共存时对蛋白结合无竞争作用。蛋白结合率对BSA浓度无依赖性,但是部分黄酮类药物蛋白结合率对药物浓度有一定的依赖性。本方法简单,可靠。
Hollow fiber-liquid phase microextraction-high performance liquid chromatography（HF-LPME-HPLC） combined with Bjerrum and Scatchard methods was applied to simultaneous and fast research of protein binding rates,protein binding constants and binding sites of flavonoids compounds.The optimal extraction conditions were polyvinylidene fluoride（PVDF） as organic solvent carrier,n-heptylalcohol as extracion phase,900 r/min of stirring rate and 2 h of extraction time.Under the optimal conditions,the protein binding rates of six flavonoids compounds,dihydromyricetin,myricetin,quercetin,kaempteride,isorhamnetin and chrysin,were 29.3%,56.8%,12.2%,25.7%,25.2% and 12.8%,respectively.Six kinds of flavonoids compounds when coexisted did not competitively combine with protein.The protein binding rates of flavonoids compounds did not depend on protein concentrations,but part of them depended on drug concentrations.This method is simple and effective.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2011年第6期857-862,共6页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.81041084)
山西省自然科学基金(No.2007011086)
山西医科大学科技创新基金资助
关键词
中空纤维液相微萃取
黄酮类化合物
蛋白结合率
蛋白结合常数
蛋白结合位点数
Hollow fiber liquid phase microextraction
Flavonoids compounds
Protein binding rates
Protein binding contants
Protein binding sites
