摘要
基于中空纤维两相液相微萃取提取新技术,建立了芝麻中共轭亚油酸的液相微萃取/高效液相色谱测定的新方法。通过研究萃取剂pH值、萃取时间和搅拌速率的影响,确定了最优化的液相微萃取条件:pH11.0的KOH溶液为萃取剂,萃取时间为30 min,搅拌速率为1 000 r/min。采用高效液相色谱法对共轭亚油酸进行定性和定量测定,结果表明,该方法的线性范围宽,相关系数(r2)为0.993,检出限(S/N=3)为17μg/L,相对标准偏差(n=6)为4.9%,共轭亚油酸的富集倍数为12.4倍。用于3种不同品牌芝麻中共轭亚油酸的分析,回收率为81.4%～94.8%。本法为植物中共轭亚油酸的分析检测提供了一种简捷有效的方法,可为乳品、植物油等其他复杂基质样品中共轭亚油酸的检测提供有效参考。
A method was developed for the determination of conjugated linoleic acid（CLA） in sesame by hollow fiber based two-phase liquid phase microextraction（LPME） coupled with high performance liquid chromatography（HPLC）.For LPME,effects of pH value of extraction solvent,extraction time and stirring rate were investigated,and the optimum experimental conditions were as follows：pH value of extraction solvent（KOH solution）：11.0,extraction time：30 min,stirring rate：1 000 r/min.The qualitative and quantitative analysis of CLA were performed by HPLC method.The proposed method provided a wide linear range with a correlation coefficient（r2） of 0.993,a detection limit（S/N=3） of 17 μg/L.The relative standard deviation（RSD,n=6） was 4.9% and the enrichment factor of CLA was 12.4.The recoveries of CLA in three kinds of sesame ranged from 81.4% to 94.8%.The method is simple and effective in the determination of CLA in plant,and could also be used in the determination of CLA in the other complex matrix samples such as dairy products,vegetable oils,etc.
出处
《分析测试学报》
CAS
CSCD
北大核心
2013年第7期877-881,共5页
Journal of Instrumental Analysis
基金
中国地质大调查项目(1212011120274)
江西省科技计划项目(20121BBG70011)
关键词
中空纤维
两相液相微萃取
高效液相色谱
芝麻
共轭亚油酸
hollow fiber
two phase liquid-phase microextraction
high performance liquid chromatography
sesame
conjugated linoleic acid
