摘要
通过对鲤鱼鳍条细胞进行原代培养,摸索出最佳的培养条件。采用组织块培养法,取一小块鳍条组织在不同培养基、温度、pH、CO2等培养条件下进行培养,对其生长状况进行观察。培养48 h可见组织块周围有细胞迁出,并形成生长晕,培养1周可形成单层细胞,主要由上皮样细胞和少量成纤维样细胞组成。本研究初步确立了鲤鱼的鳍条细胞培养条件为：培养基为M199,培养温度为25~27℃,pH为7.2~7.6,CO2浓度为5%,原代培养血清浓度为15%~20%,连续培养12 d后可得到纯化的鳍条细胞。
Through the primary culture of the carp fins cells,infered the optimum culture conditions.The major experiment method is tissue explant technique.A small piece of fins were cultivated in different conditions（media,temperature,pH,CO2）,the growth status were observed.The cells migrated out around tissues after 48 hours and the growth halo emerged.Under this condition,monolayers of primarily cultured renal cells were routinely obtained within a week,most of the cultured cells were epithelioid macrophages and little into fiber appearance cells.The optimized conditions for carp fin cells were： medium M199,25 to 27 ℃,pH 7.2 to 7.6,the concentration of CO2 was 5%,the concentration of serum for primary culture was 15% to 20%,the purification fin cells could be getten after 12 days.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第6期52-54,共3页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(30972191)
吉林省科技厅科技发展计划项目
