摘要
介绍了原代和传代异源四倍体鲫鲤(4n=200)肾组织在含20%小牛血清的M199培养基中,在25~28℃,pH7.2—8.0条件下的培养.培养的异源四倍体鲫鲤肾组织细胞保持致密单层,其主要由上皮样细胞和少量成纤维样细胞组成,染色体数为整倍体,分布于188~200之间,且多数为200.
Primary cell culture and subculture cells derived from kidney of allotetraploid hybrids are performed using M199 medium containing a moderate amount of antibiotics and 20% calf senan and incubated at temperature 25 - 28 ℃, pH 7.2 - 8.0. Most of the cultural cells are epitheial-like cells with a few fibro-blasflike cells. Chromosome number ranged from 188 to 200 with a modalnumber of 200.
出处
《湖南师范大学自然科学学报》
EI
CAS
北大核心
2006年第1期74-77,共4页
Journal of Natural Science of Hunan Normal University
基金
国家自然科学基金资助项目(30330480)