摘要
为优化‘砀山酥梨’转基因试管苗的生根条件,解决梨试管苗生根的难题,以‘砀山酥梨’转基因试管苗作为试验材料,研究了基本培养基类型、培养方式、不同的间苯三酚(PG)浓度、不同的IBA浓度和暗培养时间对生根的影响。结果表明:采用两步生根法,试管苗在附加80 mg/L PG和20 mg/L IBA的ASH基本培养基上暗培养7d后转入附加80 mg/L PG的ASH培养基上进行光照培养,生根率达到91.7%。
To optimize the conditions of rooting of in vitro transgenic Pyrus bretschneideri Rehd.cv.Dangshansuli,in the present study,the effects on the rooting of in vitro transgenic Pyrus bretschneideri Rehd.cv.Dangshansuli were assessed using different basic media,rooting method,dark culture time,and concentrations of Phloroglucinol（PG） and IBA.The results indicated that the best induction root was achieved by a two-step rooting method：firstly the plantlets in vitro were incubated on ASH medium with 80 mg/L PG and 20 mg/L IBA in the dark for 7 days;and then the shoots were transferred to the same medium with 80 mg/L PG in the normal light conditions.As a result,91.7% of the rooting rate was obtained under above conditions.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2011年第3期72-75,共4页
Journal of China Agricultural University
基金
国家"863"计划项目(2007AA10Z182)
陕西省重大科技专项(2006kz05-G4)
关键词
砀山酥梨
转基因苗
离体培养
生根
pear（Pyrus bretschneideri Rehd.cv.Dangshansuli）
transgenic plants
in vitro culture
rooting
