摘要
以‘霍巴'海棠(Malus‘Hopa')的茎尖和带腋芽的幼嫩茎段为外植体,建立了比较高效的‘霍巴'海棠组培快繁体系。结果表明:‘霍巴'海棠茎尖及茎段外植体在MS+6-BA0.1mg/L+IAA 0.2mg/L+3%蔗糖+0.48%琼脂(pH 5.8)培养基上生长状况较好;将其组培苗在WPM+6-BA 0.5mg/L+NAA 0.1mg/L+ZT 0.01mg/L+3%蔗糖+0.48%琼脂(pH 5.8)培养基上继代培养40d左右,继代增殖系数均可达到3.0左右;再经1/2 WPM+NAA0.8mg/L+1.5%蔗糖+0.48%琼脂(pH 5.8)培养基生根壮苗后,移栽成活率可达97.5%。
An efficient protocol for the rapid propagation of Malus 'Hopa'by tissue culture was developed,with the stem apex or sections as the explants.The results showed that the above explants could grow on the primary medium contained MS basal salt s and vitamins,6-BA 0.1 mg/L,IAA 0.2 mg/L and 3%sucrose and solidified w ith 0.48% agar(pH 5.8).After the shoots in vitro were subcultured for about 40 days on the subculture medium contained WPM basal salt s and vitamins,6-BA 0.5 mg/L,NAA 0.1 mg/L,ZT 0.01 and 3%sucrose and solidified with 0.48%agar (pH 5.8),the propagation coefficient by subculture could be up to 3.0 or so.Whereafter,the shoots in vitro were rooted and grew stronger on the rooting medium contained 1/2 WPM basal salts,all the vitamins of WPM,NAA 0.8 mg/L and 1.5%sucrose and solidified with 0.48%agar(pH 5.8).The survival rate of the shoots,in vitro with strong roots reached up to 97.5%after they were transplanted to soil.
出处
《北方园艺》
CAS
北大核心
2011年第10期124-127,共4页
Northern Horticulture
关键词
霍巴海棠
组织培养
快速繁殖
Malus 'Hopa'
tissue culture
rapid propagation
