摘要
[目的]建立何鲁牵牛的组培快繁体系,为其规模化生产提供理论依据。[方法]以何鲁牵牛茎段为外植体,研究何鲁牵牛不定芽增殖和生根的最适培养基。[结果]何鲁牵牛在WPM+0.3 mg/L 6-BA+0.01 mg/L NAA+0.1 g/L活性炭的启动培养基上成功诱导腋芽抽出,将腋芽转接到WPM+0.3 mg/L 6-BA+0.1 g/L活性炭培养基上进行不定芽的增殖,增殖率达4.30;在WPM+0.1 mg/L IBA+0.1 mg/L NAA+0.1 g/L活性炭培养基上生根率较高,可达90%。[结论]该研究建立了何鲁牵牛的组培快繁体系,有利于何鲁牵牛种质资源保护和工厂化生产。
[Objective] To establish tissue rapid propagation system of Ipomoea holubii,and to provide theoretical foundation for its large-scale production.[Method] Taking the stem of Ipomoea holubii as explants,we researched the optimal culture medium for adventitious buds prolifera-tion and rooting of I.holubii.[Result] Axillary buds were induced on WPM＋0.3 mg/L 6-BA ＋0.01 mg/L NAA ＋0.1 g/L active carbon （AC）.The shoots of I.holubii were cultured on WPM ＋0.3 mg/L 6-BA ＋1 g/L AC for multiplication,and the multiplication ratio was 4.30. WPM ＋0.1 mg/L IBA＋0.1 mg/L NAA＋0.1 g/L AC was the optimal medium for rooting,and the rooting rate was 90%.[Conclusion] This re-search establishes the tissue culture and rapid propagation system of I.holubii,which is helpful to the germplasm protection and industrial produc-tion of I.holubii.
出处
《安徽农业科学》
CAS
2016年第30期110-111,199,共3页
Journal of Anhui Agricultural Sciences
基金
国际先进农业科技术计划(948)项目(2011-G31)
浙江省农业科学院青年人才培养项目(2015R21R08E08)
关键词
何鲁牵牛
组培快繁
增殖
生根
Ipomoea holubii
Rapid propagation
Multiplication
Rooting
