摘要
目的 研究烧伤血清对体外培养的内皮细胞的细胞间粘附分子１（ＩＣＡＭ１）及Ｅ选择素（Ｅｓｅｌｅｃｔｉｎ）ｍＲＮＡ表达的影响，探讨实施休克期切痂上述指标的变化。 方法 Ｗｉｓｔａｒ 大鼠１７６ 只，３０％ Ⅲ度烫伤，收集不同时间点血清，采用逆转录ＰＣＲ 方法分别观察伤后不同时相点血清刺激内皮细胞ＩＣＡＭ１、Ｅｓｅｌｅｃｔｉｎ ｍＲＮＡ表达的规律。 结果 烫伤后血清可刺激内皮细胞ＩＣＡＭ１ 、ＥｓｅｌｅｃｔｉｎｍＲＮＡ表达上调，而且表达量与伤后时间呈正相关。伤后８ 及２４ 小时切痂动物血清不引起内皮细胞的上述变化，９６ 小时切痂（１－６０±０－１７）、（１－２０ ±０－０９） 效果不及休克期切痂组（０－９３ ±０－１４） 、（０－９３ ±０－２２）。 结论 及早切痂去除了坏死组织，避免了内皮细胞大量合成ＩＣＡＭ１、Ｅｓｅｌｅｃｔｉｎ 等粘附分子，减轻了由粘附分子介导的中性粒细胞对内皮细胞的进一步损害。
Objectives To examine the effect of burned serum on change in endothelial cell ICAM 1 and E selectin mRNA expression and whether these alterations can be corrected by the means of escharectomy during burn shock period. Methods One hundred and seventy six Wistar rats with 30% Ⅲ degree TBSA on back were studied. Endothelial cell ICAM 1 and E selectin mRNA were tested with the method of RT PCR. Results ICAM 1 and E selectin expression showed an increase after stimulation by serum of 4 hours post burn, reaching peak levels 168 hours after injury. ICAM 1 and E selectin expression levels could decrease to baseline within 168 hours in the rats which underwent escharectomy during shock stage, but kept higher than normal in rats without escharectomy or underwent 96 hours post burn. Conclusions These findings suggest that eschar can induce endothelial cells to express adhesion molecule. Early escharectomy is important to prevent adhesion molecule expression and SIRS.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1999年第11期692-694,共3页
Chinese Journal of Surgery
关键词
烧伤
细胞粘附分子
切痂
内皮细胞
Burns
Intercellular adhesion molecule
Escharectomy
