摘要
目的探讨微小染色体维持蛋白4(minichromosome maintenance proteins 4,MCM4)、Ki-67在卵巢浆液性肿瘤中的表达及意义。方法采用免疫组化EliVision两步法检测MCM4、Ki-67蛋白在10例正常卵巢上皮组织(对照组)、19例卵巢良性浆液性嚢腺瘤、16例交界性浆液性肿瘤和43例浆液性腺癌中的表达。结果 MCM4在对照组、卵巢良性浆液性囊腺瘤、交界性浆液性肿瘤、浆液性腺癌的阳性表达率分别为10.00%、21.05%、43.75%、79.07%,Ki-67在对照组、卵巢浆液性乳头状腺瘤、交界性嚢腺瘤、浆液性腺癌的阳性表达率分别为10.00%、15.79%、25.00%、53.49%,其随着卵巢肿瘤病变的升级呈增高的趋势。MCM4在卵巢浆液性腺癌和交界性浆液性肿瘤中的表达与正常对照组相比差异具有统计学意义(P<0.05)。Ki-67在卵巢浆液性癌和交界性浆液性肿瘤中的表达与正常对照组相比差异具有统计学意义(P<0.05)。MCM4在卵巢浆液性癌中的表达与临床分期、病理分级及转移[淋巴结转移和(或)器官转移]有明显相关(P<0.05)。Ki-67蛋白在卵巢浆液性癌中的表达与病理分级及淋巴结转移有明显相关(P<0.05),MCM4和Ki-67呈正相关。结论 MCM4、Ki-67为卵巢浆液性肿瘤的增殖指标,用于卵巢良、恶性肿瘤的鉴别和诊断,并可初步评估肿瘤预后,指导临床治疗。
Purpose To explore the expression and significance of MCM4 and Ki-67 in ovarian serous tumors.Methods MCM4 and Ki-67 expression were examined by immunohistochemistry（EliVision method）in 10 cases of control group,19 cases of benign serous tumors,16 cases of serous borderline tumors and 43 cases of serous adenocarcinomas.Results The positive expression rates of MCM4 and Ki-67 in control group,benign serous tumors,serous borderline tumors and serous adenocarcinomas were 10.00%,21.05%,43.75% and 79.07%,and 10.00%,15.79%,25.00% and 53.49%,respectively.The expression of MCM4 in borderline serous tumors and serous adenocarcinoma were significantly higher than that in control group（P0.05）.The expression of Ki-67 in borderline serous tumors and serous adenocarcinomas were significantly higher than that in control group（P0.05）.MCM4 expression was closely correlated with clinical stage,metastasis and pathological grade（P0.05）.Ki-67 expression was closely correlated with metastasis and pathological grade（P0.05）.There was significant association between MCM4 and Ki-67 expression in serous adenocarcinoma.Conclusions As the marker of cell proliferation,MCM4 and Ki-67 may be a marker for the diagnosis and differential diagnosis of benign and malignant serous tumors.MCM4 and Ki-67 may primarily assess the prognosis of the tumour and direct clinical management.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2011年第5期478-480,共3页
Chinese Journal of Clinical and Experimental Pathology
