摘要
目的：研究重组人肿瘤坏死因子（ｒｈＴＮＦ－α）对鼻咽癌细胞株ＣＮＥ３生长的抑制作用。方法：应用ＭＴＴ比色法，选择ＣＮＥ３细胞生长指数期，检测ｒｈＴＮＦ、ｒｈＩＬ－２（重组白介素－２）单用或合用在不同剂量、不同时间的抑制作用。结果：ｒｈＴＮＦ－α与ＣＮＥ３细胞培养２４ｈ，抑制率仅有９．３８％。当作用时间延长到７２ｈ，抑制率达到３０．７％。用量由４０ｕ／２×１０５细胞增加到５０００ｕ／２×１０５时，对ＣＮＥ３细胞的生长抑制率也由３．３５％增长到３３．８％。结论：ｒｈＴＮＦ－α对鼻咽癌细胞的生长的抑制作用有时相和剂量的依赖关系。ｒｈＴＮＦ与ｒｈＩＬ－２合用对ＣＮＥ３细胞生长的抑制有叠加作用。
Objective:The effects of recombinant human tumor necrosis factor α (rhTNFα) on nasopharyngeal carcinoma cell CNE 3 were investigated Methods: This study with examined the effects of rhTNF α、rhIL 2(recombinant human interleukin 2) and the combined preparation different doses and time with an MTT assay Results:The suppressive effect of rhTNF α on CNE3 cell was time and dose dependent After CNE3 cell was incubated for 72 hours and dose was increased to 5000u ,the suppressive effect of rhTNF α on CNE 3 cell was increased to above 30 7% and 33 8% respectively Combination of rhTNF α+rhIL 2 produced synergistic cell killing on CNE 3 cell line Conclusion: Our work indicated that rhTNF has a significant cell lethality against the growth of CNE 3 cell line and rhIL 2 appear to have enhanced cytotoxic activity of rhTNF α
出处
《广西医科大学学报》
CAS
1999年第5期563-565,共3页
Journal of Guangxi Medical University
基金
广西自治区科委资助
关键词
鼻咽癌
MTT比色法
RHTNF-Α
CNE3
rhTNF α(recombinant human tumor necrosis factor α)
nasopharyngeal carcinoma cell
MTT(tetrazolium based semiantomated colorimtric assay)
