摘要
目的:分析肿瘤坏死因子基因多态性分布与中国西南地区汉族人群鼻咽癌易感性的关系。方法:选择2000-10/2005-09在华西医科大学第一附属医院就诊的100例鼻咽癌患者为鼻咽癌组,均经过病理科活检确诊,其中包括未治疗44例,放疗后37例,放疗加化疗后19例,选择100名同期入院健康体检者为对照组。所有受试对象为中国西南地区汉族人,均对检测项目知情同意。采用聚合酶链反应-限制性片段长度多态性的方法检测100例中国西南地区汉族鼻咽癌患者和100名健康对照者肿瘤坏死因子α基因启动子区-308位点及肿瘤坏死因子β基因第一内含子252位点的等位基因以及基因型频率,分析两位点多态性与鼻咽癌遗传易感性的关系。结果:鼻咽癌组患者肿瘤坏死因子β(+252)位点G/A杂合子基因型频率显著高于对照组(57%,29%,P<0.01),野生型(G/G基因型)频率低于健康对照组(23%,51%,P<0.01),等位基因A的频率高于健康对照组(48.5%,13%,P<0.01);肿瘤坏死因子α(-308)位点基因型以及等位基因频率与对照组相比较无统计学差异。结论:本实验人群中未发现肿瘤坏死因子α(-308)位点多态性与鼻咽癌易感性无关;肿瘤坏死因子β(+252)位点基因多态性与鼻咽癌具有相关性,A等位基因可能是鼻咽癌的遗传易感基因,G/A杂合子基因型个体较易患鼻咽癌。
AIM： To explore the association of polymorphism distribution of tumor necrosis factor （TNF） genes with the susceptibility to nasopharyngeal carcinoma in Han population of Southwest China. METHODS： 100 patients with nasopharyngeal carcinoma treated in the First Affiliated Hospital of West China University of Medical Sciences between October 2000 and September 2005, who were diagnosed by biopsy in pathological department, of which 44 cases were not treated, 37 treated by radiotherapy, and 19 by radiotherapy and chemotherapy; at the same time, 100 healthy physical examinees were selected as control. All subjects were Han population in the Southwest of China and agreed to the detection. The allelic frequency of the TNF-α（-308） and TNF-β（＋252） loci were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） to analyze the relationship between the polymorphism of the two loci and the susceptibility to nasopharyngeal carcinoma. RESULTS： The frequency of G/A genotype of TNF-β（＋252） locus in the nasopharyngeal carcinoma patients was significantly higher than that of the control group （57%, 29%, P 〈 0.01）; that of GIG genotype was lower than the control group （23%, 51%, P 〈 0.01）; that of A allele of TNF-β（＋252） locus was higher than the control group （48.5%, 13%, P 〈 0.01 ）. There were no significant differences in the frequencies of genotype and allele of TNF-α（-308） locus between the two groups. CONCLUSION： Association is not found between the polymorphism of TNF-α（-308） locus and the susceptibility to nasopharyngeal carcinoma, but is found in TNF-β（＋252）; the A allele might be the susceptible factor in the pathogenesis of nasopharyngeal carcinoma, and people with allele G/A mutation inlocus site are susceptible to nasopharyngeal carcinoma.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第34期6819-6822,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
四川省科技攻关项目(06Z09-005)~~
