摘要
【目的】探讨木薯良种GR891的组织培养与快速繁殖技术。【方法】以越冬木薯种茎及其新长嫩茎的腋芽或顶芽为外植体,探讨不同消毒时间、6-BA和NAA浓度对木薯不定芽诱导或增殖、生根的影响。【结果】以带顶芽或腋芽的木薯幼嫩茎段作外植体进行不定芽诱导培养比较适宜;接种前采用0.1%升汞消毒10~15min,不定芽和愈伤组织诱导率均最高,分别为57.1%和61.9%。不定芽诱导培养基以MS＋0.5mg/L6-BA最佳,不定芽诱导率最高（72.2%）,芽生长较好;丛生芽增殖培养基以MS＋0.5~1.0mg/L6-BA为宜,其增殖倍数达3.0倍,并形成健壮的丛生芽;生根培养基以MS＋0.3~1.5NAAmg/L最佳,生根率可达90.0%以上。将生根苗移栽至混合基质（60%塘泥＋40%河沙）中,约1周后可萌生新根,移栽成活率达95.0%以上。【结论】在MS培养基中添加低浓度6-BA,有利于不定芽的诱导和增殖;6-BA和NAA配合使用均不利于不定芽的诱导和增殖;不定芽的诱导和生长无需添加外源NAA。0.9mg/LNAA对木薯不定芽生根效果较好,而6-BA对不定芽生根有一定抑制作用。
[Objective]The aim of this experiment was to find out a suitable method and optimal conditions for rapid micropropagation of cassava plants. [Method]The axillary or apical buds of stems derived from over wintered cassava plants and its new plants were used as explants for induction, multiplication and rooting of adventitious buds under different conditions, viz., different sterilization times and concentrations of 6-BA and NAA. [Result]As compared to the over wintered stem of cassava, the young stem derived from new plant was more suitable for the induction of adventitious buds of cassava. When the explants were sterilized with HgCl2 （0.1%） solution for 10-15 min before inoculation to the medium, the adventitious bud and callus showed the highest induction rate （57.1 and 61.9%, respectively）. The best medium for induction of axillary buds was MS medium containing 0.5 mg/L 6-BA, which attained 72.2% induction rate of adventitious buds. The suitable medium for multiplication of cluster buds was MS ＋ 0.5-1.0 mg/L 6-BA, 2-3 times multiplication in this medium made the cluster buds more stronger. The MS medium containing 0.3-1.5 mg/L NAA was recorded as best medium for rooting of adventitious buds and resulted in 90.0% rooting rate. The survival rate of rooted plantlets was recorded as 95.0% when plantlets were transferred in pots containing 60.0% pond mud and 40.0% river sand. [Conclusion]Adding low concentration of 6-BA in MS medium was beneficial to the induction and multiplication of adventitious buds of cassava, while the induction and growth of adventitious buds did not require NAA. The combination of 6-BA and NAA was not found beneficial for induction and multiplication of adventitious buds. Addition of 0.9 mg/L NAA in MS medium showed better effects on rooting of adventitious buds, while adding 6-BA in medium played an inhibitory role in rooting.
出处
《南方农业学报》
CAS
CSCD
2011年第5期471-474,共4页
Journal of Southern Agriculture
关键词
木薯
种茎
顶芽
腋芽
不定芽
诱导
增殖
cassava
stem
apical bud
axillary bud
adventitious bud
induction
multiplication
