摘要
以木薯（ＭａｎｉｈｏｔｅｓｃｕｌｅｎｔａＣｒａｎｔｚ）品种“南植１８８”的嫩叶为外植体，用１０～８０ｍｇ／ＬＮＡＡ能直接诱导初生体细胞胚胎发生和芽的形成。与用４ｍｇ／Ｌ２，４Ｄ诱导木薯嫩叶产生初生体细胞胚及其植株再生相比，ＮＡＡ具有以下特点：它不仅能直接诱导初生体细胞胚发生，而且能直接诱导芽的形成；而２，４Ｄ只能诱导体细胞胚胎发生而不能诱导芽的形成。ＮＡＡ诱导初生体细胞胚较快，在培养９～１３ｄ后就能见到初生体细胞胚的形成，在培养１０～１４ｄ就能见到芽的形成；而２，４Ｄ所诱导的体细胞胚至少在培养１５ｄ后才能见到。ＮＡＡ诱导的初生体细胞胚的植株再生频率可高达４８％；而２，４Ｄ所诱导的体细胞胚的再生频率只有４．１％。实验结果还证实，在供试的另外１６个木薯品种中，有１２个品种能够直接从嫩叶诱导初生体细胞胚胎发生或芽的形成。
Primary somatic embryogenesis and shoot organogenesis in vitro could be directly induced from immature leaves of cassava (Manihot esculenta Crantz) with higher concentration (10 to 80 mg/L) of NAA. Compared with 4 mg/L 2,4D on the induction and regeneration system, NAA showed some advantageous characteristics, that is, NAA could directly induce both primary somatic embryogenesis and shoot organogenesis, whereas 2,4D could only induce somatic embryogenesis. NAA induced somatic embryogenesis much quicker, producing visible somatic embryos within 9 to 13 days and shoot (tips) within 10 to 14 days, than 2,4D, which would induce visible somatic embryos after 15 days. Plant regeneration from the NAAinduced somatic embryos was as high as 48%, but was only 4.1% from that of 2,4D. The test also showed that primary somatic embryogenesis or shoot organogenesis could be induced directly from immature leaves in 12 out of 16 cassava varieties.
基金
广东省自然科学基金
关键词
木薯
嫩叶
NAA
初生体细胞
胚胎发生
芽形成
Manihot exculenta, Immature leaf, NAA, Primary somatic embryogenesis, Shoot organogenesis
