摘要
以黑松带子叶顶芽为外植体建立了包括丛生芽诱导、伸长和生根的植株再生体系。结果表明：培养基中激素的种类、浓度以及外植体年龄对丛生芽的诱导有较大影响。单独使用6-BA能诱导产生丛生芽，但数量少，生长慢，NAA与6-BA配合使用能取得更好的效果。在试验的所有组合中，4．0mg／L6-BA＋0．05mg／LNAA效果最佳。截取黑松外植体的最佳苗龄为22～28d。已分化的丛生芽在无6-BA但附加0．1mg／LNAA的改良GD培养基中伸长较明显，GA3不能促进黑松丛生芽的生长且有毒害作用。将伸长的丛生芽接种在附加0．2mg／LNAA的1／2WPM培养基上培养14d再转移至无激素的1／2WPM中，1个月后，生根率达23．3％。
A plantlet regeneration protocol was developed for Pinus thunbergii. The procedure involves 3 steps, axillary buds induction, buds growth and root induction. Axillary buds induction was found to be influenced by the concentration of 6-benzyladenine （6-BA） and alpha-naphthylacetic acid （NAA） in the induction medium as well as the age of the seedling from which the explant was taken. Of the various 6-BA and NAA combination tested, modified Gresshoff and Doy＇s medium （GD） with 4 mg/L 6-BA and 0.05 mg/L NAA proved to be the most effective, and the right stage of seedling was 22-28 d. During the next 5 weeks of culture on the same 6-BA-free but with 0.1 mg/L NAA medium multiple shoots elongated from the buds. The medium containing GA3 did not support buds growth. Elongated shoots were transferred to half-strength WPM medium supplemented with 0.2 mg/L NAA for root initiation and 14 days later transferred to half-strength WPM medium without any hormone for root development. After 1 month, 23.3% of the shoots had formed adventitious roots.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第3期27-31,共5页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家林业局"948"项目(2003-4-38)
关键词
黑松
实生苗
丛生芽诱导
植株再生
Pinus thunbergii
Seedling
Axillary bud induction
Plantlet regeneration
