摘要
目的研究杀白细胞素(PVL)pvl基因阴性耐甲氧西林金黄色葡萄球菌(MRSA)对多种抗菌药物的耐药性。方法采用头孢西丁纸片扩散法检测MRSA;用聚合酶链反应(PCR)检测m ecA基因及pvl基因;采用琼脂稀释法测定金黄色葡萄球菌最低抑菌浓度(M IC)并检测pvl基因阴性MRSA对12种抗菌药物的耐药性。结果 71株金黄色葡萄球菌中检测出33株pvl基因阴性MRSA,检出率为46.4%(33/71);对青霉素类抗菌药物的耐药率为100.0%;对其他类抗菌药物呈多重耐药并以ECLOTGMP为主要耐药模式,占66.7%(22/33);未发现对达托霉素、替考拉宁和万古霉素耐药的菌株。结论医院感染中以pvl基因阴性MRSA为主,并呈现多重耐药;pvl基因阴性MRSA主要耐药模式为对红霉素、环丙沙星、克林霉素、苯唑西林、四环素、庆大霉素、复方磺胺甲口恶唑和青霉素耐药,是主要流行株,要谨慎使用以上8种抗菌药物。
Objective To investigate the resistance of panton-valentine leukocidin（PVL） pvl gene-negative methicillin-resistant Staphylococcus aureus（MRSA） to antimicrobial drugs.Methods Cefoxitin disk diffusion test was used to detect MRSA.Polymerase chain reaction（PCR） was used to the detection of mecA gene and pvl gene.The minimum inhibitory concentration（MIC） of MRSA was detected by agar dilution,and the resistance of pvl gene-negative MRSA to 12 antimicrobial drugs was detected.Results 33 pvl gene-negative MRSA were detected from 71 strains of Staphylococcus aureus.The detection rate was 46.4%（33/71）.The resistance rate of penicillin was 100%,and other antimicrobial drugs showed multiple drug resistance.The main resistance patterns accounting for 66.7%（22/33） was showed the resistance of ECLOTGMP.The resistant strains to daptomycin,teicoplanin and vancomycin were not found.Conclusions The pvl gene-negative MRSA with resistance to erythromycin,ciprofloxacin,clindamycin,tetracycline,gentamicin,sulphamethoxazole,oxacillin and penicillin may be the most popular,which is the multidrug resistance pattern of major epidemic strains.These 8 antimicrobial drugs should be used carefully.
出处
《检验医学》
CAS
北大核心
2011年第5期295-298,共4页
Laboratory Medicine
基金
上海市闵行区科委资助项目(2005-05)
关键词
杀白细胞素
耐甲氧西林金黄色葡萄球菌
耐药性
Panton-valentine leukocidin
Methicillin-resistant Staphylococcus aureus
Resistance
