摘要
目的建立小鼠子宫内膜损伤模型。方法借鉴热球子宫内膜去除术原理,利用宫腔导管贯穿小鼠Y型宫腔的一侧,给予预温85℃流动热水经80 s热损伤小鼠子宫内膜组织,达到子宫内膜广泛剥脱目的。结果处理后48 h宫腔内见子宫内膜层广泛脱落,部分子宫腔壁内膜完全脱落,内膜间质可见大量血管充血;处理后7 d子宫腔及浅肌层大量的炎症细胞浸润,未见明显的腺体结构;处理后14 d子宫腔形态缩小或闭锁,未见正常内膜,并有炎症细胞浸润和纤维化表现,子宫外形尚存在。合笼后,模型小鼠仍能受孕,但胚胎仅存在于未损伤侧子宫腔。结论利用宫腔导管对小鼠子宫腔进行热处理,初步建立了子宫内膜损伤的动物模型。
Objective Established mice endometrial damage model.Methods Reference the principle of thermal balloon endometrial ablation,use of intrauterine catheter through the right side of mice y-shape uterine cavity,give advance temperature 85 ℃ flow hot water by 80 s heat damage mice endometrial organization.Results Treatment after 48 h,uterine cavity see endometrial layer falls off,part uterine cavity wall lining fall off completely,endometrial interstitial visible abundant vascular congestion,after processing 7 d uterine cavity and shallow muscularis lots of inflammatory cells infiltration,did not see the obvious glandular structures,the processed uterine cavity form 14 d to narrow or atresia,did not see the normal endometrium,and inflammatory cells infiltrating and fibrosis performance,uterine shape it still exists.Co-cage,damage did not affect mice embryos conceived,but can still exist in not damaged side uterine cavity.Conclusion The animal model of endometrial damaged can be established by thermal damage.
出处
《福建医科大学学报》
2011年第1期34-36,44,共4页
Journal of Fujian Medical University
基金
福建省自然科学基金(2010J01155)
关键词
子宫内膜
疾病模型
动物
高温
诱发
Endometrium
Disease Models
Animal
Hyperthermia
Induced
