摘要
以八月红(Pyrus communis L.‘Bayuehong’)×砀山酥梨(P.×bretshneider‘Dangshansuli’)F1代杂交分离群体(共118株)为试材,测定了成熟果实的酸含量,从表型上分析了梨果实含酸量的遗传规律。利用225对源自梨和苹果基因组的SSR引物,结合分离群体分离分析法(Bulked Segregant Analysis,BSA)和选择基因型分析(Selective geno-typing,SG)法,对酸/低酸性状进行群体分离分析,结果表明,标记KU10与果实酸/低酸性状连锁,遗传距离为12.15cM,并由此将果实酸/低酸性状的QTL初步定位到梨遗传图谱的第2连锁群上。表型分析和标记分析结果表明,梨果实酸性状对低酸性状为显性,受加性效应和环境因素的影响,同时存在一定的非加性效应。
The inheritance of the fruit acid trait as titratable acid of mature fruit was analyzed using the F1 segregation population（total of 118 seedlings） derived from the cross Bayuehong（Pyrus communis L.） × Dangshansuli（P.× bretshneider Rehd.）.Meanwhile,225 pairs of SSR primers originated from pear and apple genome were screened by bulked segregant analysis（BSA） and selective genotyping（SG）.Of those primer pairs,only primer KU10 produce a polymorphic band linked to acid trait,the genetic distance of the molecular marker is 12.15 cM,and the quantitative trait loci（QTL） analysis of fruit acid/low-acid trait was mapped on second linkage group（LG2） of the pear genetic map.The comprehensive analysis of molecular marker and phenotype revealed that the fruit acid trait is dominant to low-acid trait and is affected by both additive gene effect and environmental factors,and it is also affected by non-additive gene effect.
出处
《果树学报》
CAS
CSCD
北大核心
2011年第3期389-393,共5页
Journal of Fruit Science
基金
国家高技术研究与发展计划重点项目(2006AA100108)
