摘要
以‘大久保’与‘兴津油桃’杂交的F2 代 10 9株群体为试材 ,采用AFLP、RAPD、SSR分子标记进行遗传分析。筛选扩增稳定、多态性丰富的 36对AFLP引物、 3对SSR引物、 2对RAPD引物进行群体分离分析 ,获得分离标记 136个 ,卡方检验 2 7个标记偏离孟德尔分离比例。应用Mapmaker分析软件将符合孟德尔遗传分离比例的标记构建了包含 11个连锁群的连锁图谱 ,每个连锁群包含 3～ 2 1个标记 ,平均为 8 73个标记。该图谱覆盖基因组 10 6 1 8cM ,11个连锁群的平均长度为 96 5cM ,标记间平均图距为11 0cM ,与果实毛 /油桃 (G/g)、白 /黄肉 (Y/y)连锁的RAPD标记、非酸 /酸 (D/d)性状连锁的AFLP标记分别定位在第 3、 7、 9连锁群上。
A linkage map of peach was constructed with AFLP, SSR and RAPD markers in 109 F_2 population derived from the cross of‘Okubo’and‘Xingjin’ nectarine. The selected primers including 36 AFLP, 3 SSR and 2 RAPD with rich polymorphism and steady bands were tested in progeny. 136 markers were scored , of which 27 markers were skewed segregation by X2 test. Mapmaker were used to assigned the expected segregation markers to 11 linkage groups, the number of loci mapped in the 11 linkage group was 3-21 and there was 8.73(average value)loci per group. Whole length of linkage map was 1061.8 cM, The average length of 11 groups was 96.5 cM, and average interval between markers was 11.0 cM. RAPD markers linked to G/g, Y/y and AFLP markers linked to D/d gene were located in group 3、group 7 and group 9 respectively.
出处
《园艺学报》
CAS
CSCD
北大核心
2004年第5期593-597,共5页
Acta Horticulturae Sinica
基金
国家自然科学基金资助项目 (3 0 2 70 92 1)
北京科技合同项目 (95 483 0 3 0 0 )
北京市科技新星计划资助项目 (95 3 82 2 0 0 )
关键词
桃
分子连锁图
构建分析
遗传分离比例
Peach〔Prunus persica(L.) Batsch〕
Linkage map
AFLP
RAPD
SSR$$$$
