摘要
目的建立骨风宁胶囊中重楼皂苷Ⅰ和重楼皂苷Ⅱ含量的分析方法。方法采用C18色谱柱,以0.4%磷酸:乙腈(55∶45)为流动相,检测波长210nm,流速1mL.min-1,柱温40℃。结果在0.09816～0.9816mg.mL-1范围内,重楼皂苷Ⅰ的峰面积与浓度有良好的线性,r=0.9999;在0.09889～0.9889mg.mL-1范围内,重楼皂苷Ⅱ的峰面积与浓度有良好的线性,r=0.9998。重楼皂苷Ⅰ和重楼皂苷Ⅱ的平均回收率为98.00%和98.90%。结论该方法操作简便,重现性好,能有效监控骨风宁胶囊的质量。
OBJECTIVE To establish the determination method for Paridis saponinⅠand Paridis saponin Ⅱ in Gufengning capsule.METHODS Kromasil C18 column（4.6 mm×250 mm,5μm）was used;0.4% phosphoric acid：Acetonitrile（55∶45）was used as a mobile phase;Flow rate was 1mL·min-1;Detection wavelength was 210 nm,column temperature was 40℃;RESULTS the Paridis saponinsⅠconcentration and peak area was showed good linear relationship between 0.09816～0.9816mg·mL-1,r=0.9999.The Paridis saponinsⅡconcentration and peak area was showed good linear relationship between 0.098891～0.9889mg·mL-1,r=0.9998.The paridis saponins Ⅰand paridis saponinsⅡrecovery of the added sample was 98.00% and 98.90% separately.CONCLUSION The method showed good precision and reproducibility,the result is accurate,can be used for Paridis saponinⅠand Paridis saponinⅡof Content determination in Gufengning capsule.
出处
《海峡药学》
2011年第5期58-60,共3页
Strait Pharmaceutical Journal
