摘要
目的:制备重楼总皂苷长循环脂质体,并对其进行表征。方法:以粒径、Pd I、Zeta电位及包封率为评价指标,考察制备重楼总皂苷长循环脂质体的各个因素。并采用Malvern粒度仪测定脂质体的粒径分布、Pd I及Zeta电位,透射电镜考察其形态,并考察长脂质体的稳定性。结果:重楼总皂苷长循环脂质体的平均粒径为(109.4±32.7)nm,Pd I为(0.171±0.036),Zeta电位为(-36.7±4.5)m V,包封率为(93.5±3.2)%;透射电镜显示脂质体粒径均一,成球状分布;长期稳定性研究表明,长循环脂质体在4℃条件下放置3个月稳定。结论:重楼总皂苷长循环脂质体制备工艺简单易行,可以得到包封率高、稳定性好的脂质体制剂。
Objective： To prepare and characterize the PEGylated liposomes containing total saponins of Paris Polyphylla. Meth- ods： Using the size, PDI, zeta potential and encapsulation efficiency of the liposomes as the indicators, the influencing factors in the preparation were optimized. The particle size, PDI and zeta potential were studied by a Malvern Zetasizer, the morphology was ob- served under a TEM, and the stability was studied as well. Results： The particle size, PDI, zeta potential and encapsulation efficiency of the PEGylated liposomes was （109.4 ±32.7） nm, （0. 171 ±0.036）, （ -36.7 ±4.5） mV and （93.5 ±3.2） %, respectively. The liposomes were small spheres with smooth surface under the TEM. The long term stability studies showed that the liposomes were stable in 3 months after stored at 4 ℃. Conclusion ： The preparation technology of the PEGylated liposomes containing total saponins of Paris Polyphylla is feasible, which can obtain liposomal preparations with high entrapment efficiency and good stability.
出处
《中国药师》
CAS
2015年第6期907-912,共6页
China Pharmacist
基金
陕西教育厅项目(编号:2013JK0816)
关键词
重楼总皂苷
长循环脂质体
稳定性
Total saponins of Paris Polyphylla Smith
PEGylated liposomes
Stability
