摘要
目的：建立同时测定彝肤软膏中重楼皂苷Ⅰ、重楼皂苷Ⅱ高效液相色谱分析方法。方法：采用高效液相色谱法对彝肤软膏中重楼皂苷Ⅰ、重楼皂苷Ⅱ同时进行含量测定：Hypersil ODS2（4．6mm×150mm，5μm）；预柱：IBBM-612111，C-18柱芯；乙腈-0．02％磷酸（43：57）为流动相，流速1mL·min^-1，检测波长203nm，柱温40℃。结果：重楼皂苷Ⅰ在0．1024～3．2μg线形关系良好，r=0．9998，平均回收率97．4％，RSD1．8％（n=6）；重楼皂苷Ⅱ在0．064—2．0μg线形关系良好，r=0．9999，平均回收率101．4％，RSD1．0％（n=6）。结论：本方法准确可靠、重复性好，能有效控制该制剂的质量。
Objective： To develop a method for the determination of Chonglou saponin Iand Chonglou saponin II in Yifu ointment. Method： The chromatographic separation was performed on Hypersil ODS2 C18 column （4. 6 mm × 150 mm, 5 μm） and IBBM-612111 ODS C18 guard column. Acetonitrile-0. 02% phosphoric （43： 57） as mobile phase. The flow rate was 1 mL · min^-1 and column temperature was set at 40 ℃. The UV detection wavelength was set 203 nm. Result： Chonglou saponinIshowed a good linear relationship at a range of 0. 102 4-3. 2 μg, r =0. 999 8, the average recovery was 97. 4%, and RSD was 1.8% （ n = 6） ; Chonglou saponin Ⅱ showed a good linear relationship at a range of 0. 064-2. 0 μg, r =0. 999 9, the average recovery was 101.4%, and RSD was 1.0% （n =6）. Conclusion： The method is accurate with the good reproducibility and can be used for the quality control of Yifu Ointment.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第22期2368-2370,共3页
China Journal of Chinese Materia Medica
基金
四川省中医药管理局民族药重点项目(2004A09)
关键词
彝肤软膏
重楼皂苷Ⅰ重楼皂苷Ⅱ
含量测定
Yifu ointment
chonglou saponin Ⅰ
chonglou saponin Ⅱ
quantitative detemination
