摘要
以ODS-C18（5μm,4.6 mm×150 mm）为色谱柱,庚烷磺酸钠离子对试剂（PIC-B7）和甲醇为流动相并采用梯度洗脱,在流速为1 mL.min-1、柱温为30℃条件下,用波长为275 nm的紫外检测器进行高效液相色谱法（HPLC）检测和分析白牦牛乳中B族维生素的含量.结果表明：白牦牛乳中维生素B3的含量最高（345.588 6μg.（100 mL）-1）,维生素B11的含量最低（4.815 7μg.（100 mL）-1）;B族维生素的平均回收率为82.98%~97.96%.在本研究所建立的样品前处理方法和HPLC分析色谱条件下,能快速、准确地同时测定白牦牛乳中各种B族维生素的含量,具有重现性好和回收率高的特点.
The high performance liquid chromatography method for a simultaneous contents determination of B vitamins in white yak milk had been developed.Separation and contents determination of B vitamins were achieved by using ODS-C18 column（5 μm,4.6 mm×150 mm）,gradient elution with sodium heptanesulfonate（PIC-B7 reagent） and methanol as mobile phase at the flow of 1 mL·min-1,column temperature 30 ℃ and 275 nm UV.The results showed that the maximal and minimal contents of B vitamins were B3（345.588 6 μg·（100 mL）-1） and B11（4.815 7 μg·（100 mL）-1） respectively.The average recover rate of B vitamins was 82.98 %～97.96 %.With the ascertained samples pre-dispose method and chromatography conditions in the study,B vitamins in white yak milk have been simultaneous determined quickly and accurately,and with the advantages of better repeatability and higher recovery.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2011年第2期119-123,共5页
Journal of Gansu Agricultural University
基金
教育部优秀青年教师科研和奖励基金(0330872)
关键词
白牦牛乳
B族维生素
高效液相色谱
white yak milk
B vitamins
high performance liquid chromatography（HPLC）
