摘要
本文研究了分布于西藏和四川地区的藏山羊乳蛋白、乳糖和乳脂含量,并对乳中4种酶活力及乳清蛋白的组成进行了研究。结果表明:藏山羊乳中3种营养成分存在一定的地区差异,脱脂乳中乳酸脱氢酶,碱性磷酸酶,γ-谷氨酰转肽酶、乳过氧化物酶活力分别为81.9±37.8、7.7±3.5、437.4±93.0、532.7±202.6单位。乳过氧化物酶在体外可被10^(-5)M的硫氰酸根或乳酸根活化。用琼脂糖凝胶电泳可将乳LDH分出LDH_1、LDH_2和LDH_3三带,各带所占百分比例分别为50.6±4.5、31.6±4.2和17.8±3.3,提示乳LDH不是来源于血液。用SDS—PAGE可将乳清蛋白分出5条区带。
The results showed that the concentrations of protein, lactose and fat in the milk of Tibcfan goats were different in three regions. The activities of lactate dehydrogenase, Alkaline phosphatase, I-Glu-tamyl transpeptidase and Lacto-peroxidase in the skim milk were 81. 9±37. 8,7. 7±3. 5,437. 4±93. 0 and532.7±202. 6 respe-ctively.
Lactoperoxidase in the milk was activited by 10-5mol/L thiocyan ate or lactate in vitro. LDH was divided into LDH1 (50. 6±4. 5%),LDH2(31. 6±4. 2%),LDH3(17. 8± 3. 3%) by agrose gel elecphoresis, Suggesting that LDH in the milk did not come from the blood, Whey proteins were studied by means of SDS-PAGE, and 5 protein bands were observed.
出处
《中国畜牧杂志》
CAS
北大核心
1993年第5期10-12,共3页
Chinese Journal of Animal Science
基金
国家自然科学基金资助项目
