摘要
目的:探讨外源性亮菌抗顺铂(DDP)化疗过程中并发胃平滑肌电活动改变及5-羟色胺(5-HT)在此过程中的调控作用.方法:90只♂SD大鼠随机分为6组:空白对照组、模型组、亮菌低、中、高剂量组及昂丹司琼组,参照DeJonghe法以6mg/kg体质量腹腔注射DDP制造化疗性大鼠异食癖恶心呕吐模型,采用Biopac专用胃肠电记录分析系统检测胃窦部平滑肌电活动,运用ELISA方法检测大鼠胃窦5-HT含量,观察亮菌对DDP复制模型后不同时间段的胃电活动的影响及其与5-HT的关系.结果:造模后24-72h,模型组大鼠胃窦慢波每分钟频率(CPM)与平均振幅(AV)均显著高于空白对照组(P<0.05)与亮菌中、高剂量组(P<0.01),以造模后24h模型组(CPM:7.33±2.92,AV:249.75±79.09)与空白对照组(CPM:3.00±1.55,AV:148.04±63.51)、亮菌高剂量(CPM:4.13±1.14,AV:163.46±26.14)的差异最明显.与空白对照组相比较,其他各组大鼠胃窦组织5-HT含量(μg/L)均明显升高(24h:389.7±25.5,354.5±34.9,314.5±31.5,282.2±19.6,271.0±21.1vs244.6±27.3,P<0.05或0.01).胃窦CMP及AV总体趋势随着5-HT含量增加而上升,尤以造模后24h趋势最为明显.结论:亮菌可有效抑制DDP化疗所诱发的胃节律性运动紊乱,其对化疗过程中诱发的胃节律性运动紊乱的抑制作用机制可能与5-HT的调控作用有关.
AIM：To investigate the preventive effect of Armillariella tabescens against cisplatin-induced changes in gastric electromyographic activity in rats and to explore the role of 5-hydroxytryptamine（5-HT） in this process.METHODS：Ninety male Sprague-Dawley rats were randomized into control group,model group,ondansetron group,low-,medium-,and high-dose Armillariella tabescens groups.Rats were injected intraperitoneally with cisplatin（6 mg/kg） to induce pica.The electrical activity of gastric antral smooth muscle was recorded and analyzed using the Biopac MP100-CE acquisition system.The concentration of 5-HT in gastric antrum tissue was assayed by ELISA.The effect of Armillariella tabescens on gastric electromyographic activity and its relationship with 5-HT were then examined.RESULTS：During 24-72 h after cisplatin administration,the cycles per minute（CPM） and amplitude of vibration（AV） of slow-wave in the gastric antrum were significantly higher in the model group than in the control group（all P 0.05）,medium-,and high-dose Armillariella tabescens groups（all P 0.01）,and the differences were most signif icant between the model group（CPM：7.33 ± 2.92,AV：249.75 ± 79.09） and the control group（CPM：3.00 ± 1.55,AV：148.04 ± 63.51） and high-dose Armillariella tabescens group（CPM：4.13 ± 1.14,AV：163.46 ± 26.14） at 24 h after cisplatin administration.Compared with the control group,the concentrations of 5-HT（μg/L） in the other groups were dramatically increased（24 h：389.7 ± 25.5,354.5 ± 34.9,314.5 ± 31.5,282.2 ± 19.6,271.0 ± 21.1 vs 244.6 ± 27.3,all P 0.05 or 0.01）.CMP and AV increased with the increase in the concentrations of 5-HT.CONCLUSION：Armillariella tabescens could effectively inhibit cisplatin-induced changes in gastric electromyographic activity in rats possibly via mechanisms involving 5-HT.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第6期562-567,共6页
World Chinese Journal of Digestology
基金
国家中医药管理局中医肿瘤病学重点学科基金资助项目
No. 国中医药发(2009)30~~
关键词
亮菌
胃电图
5-羟色胺
化学治疗
呕吐
Armillariella tabescens
Electrogastrogram
5-Hydroxytryptamine
Chemotherapy
Vomiting
