摘要
采用邻苯三酚自氧化法、Fenton反应体系法、DPPH体系法,以抗坏血酸为阳性对照,对亮菌多糖-1b(ATPS-1b)清除超氧阴离子自由基(O2-·)、羟自由基(·OH)和二苯代苦味肼基自由基(DPPH·)的效果进行了测定。结果表明ATPS-1b对O2-·、·OH和DPPH·3种自由基均具有很强的清除作用,且存在良好的量效关系,随着ATPS-1b浓度的增大,其清除效果增强;清除O2-·、·OH、DPPH·3种自由基的EC50值分别为0.69mg·mL-1、8.73mg·mL-1、2.01mg·mL-1;对3种自由基的最大清除率分别为86.17%、76.4%、78.52%;ATPS-1b具有显著的体外抗氧化活性。
The effects of polysaccharide-1b from Armillariella tabescens（ATPS-1b） to remove superoxide anion free radical, hydroxyl free radical and DPPH- free radical were determined by the methods of the auto-oxidation of pyrogallol system, Fenton reaction system and DPPH. system. The results showed that ATPS-1b had strong scavenging capacity to O2^-, ·OH and DPPH.. Furthermore, scavenging capacity and anthocyanins concentration showed a position of dose-response relationship. The three free radicals had strong scavenging resuhs with＇the increasing of coneenteation. The EC50 of scavenging free radicals were 0.69 mg· mL^-1, 8.73 mg·mL^-1, 2.01 mg. mL^-1 respectively and maximum scavenging rates were 86.17%, 76.4%, 78.52%. ATPS-1b had significant antioxidant activity in vitro experiments.
出处
《中国食用菌》
2008年第6期38-40,共3页
Edible Fungi of China
关键词
亮菌
多糖
超氧阴离子自由基
羟自由基
二苯代苦味肼基自由基
抗氧化活性
Armillariella tabescens
Polysaccharide
Superoxide anion free radical
Hydroxyl free radical
DPPH, free radical
Antioxidant activity
