摘要
用DPPH.自由基检测法测定了川桂叶总黄酮清除自由基的生物活性。在波长为517 nm,反应时间45min的条件下测定了川桂叶总黄酮、芦丁和二丁基羟基甲苯(BHT)清除DPPH自由基的EC50值。结果表明,川桂叶总黄酮对DPPH.自由基具有明显的清除作用,川桂叶总黄酮、芦丁和BHT清除DPPH.自由基的EC50值分别为4.145 mg/L、9.650 mg/L、37.91 mg/L,清除DPPH.自由基能力的大小顺序为川桂叶总黄酮>芦丁>BHT。
The total flavonoids were extracted from Cinnamomum Wilsonii Gamble leaves.Their radical scavenging activities were determined by using DPPH radical scavenging method.EC50 values of the total flavonoids from Cinnamomum Wilsonii Gamble leaves,Rutin and BHT on DPPH radical scavenging were determined under wavelength of 517 nm and reaction time of 45 min.The results showed that the total flavonoids from Cinnamomum Wilsonii Gamble leaves have more significant effects on DPPH radical scavenging.EC50 values of the total flavonoids from Cinnamomum Wilsonii Gamble leaves,Rutin and BHT are 4.145 mg/L,9.650 mg/L and 37.91 mg/L,respectively.The sequence of radical scavenging ability is the total flavonoids〈Rutin〉BHT.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2010年第10期125-128,132,共5页
Journal of Central South University of Forestry & Technology
基金
湖南省教育厅基金资助项目(07C811)
关键词
应用化学
川桂
叶
总黄酮
二苯代苦味肼基自由基
applied chemistry
Cinnamomum wilsonii Gamble
leaves
total flavonoids
2
2-diphenyl-1-picrylhydrazyl radical
