摘要
通过不同方式处理脱酚葡萄籽粉,考察了多肽和多酚含量的变化及其抗氧化性。结果表明:先培养地衣芽孢杆菌使其产碱性蛋白酶达到一定程度后,加入一定固液比的脱酚葡萄籽粉,得到的多肽和多酚量达最大值,分别为4.23g/L和128.83mg/L。脱酚葡萄籽粉水解后的多肽类物质对羟自由基和DPPH自由基的清除起关键作用,而且多肽类物质的组成可能比较复杂。脱酚葡萄籽粉中非碱性蛋白酶的水解成分在清除超氧阴离子自由基方面发挥主要作用,且地衣芽胞杆菌的作用使其对超氧阴离子自由基的清除率提高了约20%。
Through different methods to deal with grape seed powder removed polyphenols, the quantity of peptides and polyphenols and its antioxidative activities was inspected.Firstly, bacillus licheniformis was cultivated to make the alkaline protease reach to a certain amount.Then grape seed powder of designated ratio was inoculated,which was the optimum process mode. The results indicated that peptides and polyphenols concentration up to the maximum 4.23g/L and 128.83mg/L.In terms of antioxidant, peptides played a key role on eliminating the hydroxyl free radicals and DPPH free radicals,and the composition of peptides might be more complicated.The clearance ability to superoxideanion free radicals should contribute to the water soluble materials of the grape seed powder, but the hydrolysate of alkaline protease also increased clearance rate about 20%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第21期87-91,共5页
Science and Technology of Food Industry
基金
河西学院大学生科技创新项目
甘肃省高校省级重点实验室项目(XZ1015)
关键词
脱酚葡萄籽粉
地衣芽孢杆菌
多肽
抗氧化活性
grape seed powder removed polyphenols
Bacillus licheniformis
peptides
antioxidative activities
