摘要
目的:观察亮菌多糖IPS-B2对巨噬细胞分泌细胞因子、一氧化氮(NO)生成以及巨噬细胞中白介素-1β(IL-1β),白介素-6(IL-6),肿瘤坏死因子-α(TNF-α)和NO合成关键酶诱导型一氧化氮合酶(iNOS)基因转录的影响。方法:采用ELISA和Griess法检测IPS-B2对小鼠腹腔巨噬细胞生成细胞因子IL-1β,IL-6,TNF-α和细胞毒效应分子NO的影响;采用SYBR GreenⅠ指示的Real-time RT-PCR技术研究IPS-B2对小鼠腹腔巨噬细胞中IL-1β,IL-6,TNF-α和NO合成关键酶iNOS基因在mRNA转录水平上的作用。结果:IPS-B2能显著提高小鼠腹腔巨噬细胞培养上清液中IL-1β,IL-6,TNF-α和NO的含量,增强IL-1β,IL-6,TNF-α和NO合成关键酶iNOS基因的转录水平。结论:IPS-B2可能是通过提高巨噬细胞分泌生物活性物质,并促进此类生物活性物质的基因转录,从而实现其抗肿瘤的重要作用。
Objective： To observe the effect of IPS-B2 on mouse peritoneal macrophages and the transcription of IL-1β, IL-6, TNF-α and iNOS. Method： ELISA method and Griess method were used to detect the effect of mouse peritoneal macrophages produce cytokines IL-1β, IL-6, TNF-α and cytotoxic effectors NO. The transcription of IL-1β, IL-6, TNF-α and iNOS was detected by realtime RT-PCR method. Result： IPS-B2 could not promote mouse peritoneal macrophage production, but it could significantly improve the IL-1β, IL-6, TNF-α content in mouse peritoneal macrophages culture supernatant, and increase the gene expression of IL-1β, IL- 6, TNF-α and iNOS. Conclusion： IPS-B2 can enhance the ability of peritoneal macrophages to excrete bioactive substances and promote the transcription of bioactive substances to antitumor.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第11期1305-1308,共4页
China Journal of Chinese Materia Medica
