摘要
[目的]为葡萄试管苗培养过程中的光照和氮素管理提供理论依据。[方法]以红地球葡萄试管苗为试材,研究了不同光照强度和NH4+-N、NO3–-N不同配比对葡萄试管苗的生长发育和生理特性的影响。[结果]随氮源中NH4+-N所占比例增加,葡萄试管苗叶绿素含量也相应增加。在光强为5 000 lx、NH4+-N∶NO3–-N为5∶5条件下生长的葡萄试管苗,叶绿素含量最高。在相同氮源条件下生长的试管苗,可溶性糖和可溶性蛋白质含量随光照强度的增加而升高。当光照为5 000 lx、NH4+-N∶NO3–-N为0.8∶9.2(改良B5培养基配方氮素比例)时,葡萄试管苗可溶性糖和可溶性蛋白质含量最高。[结论]在光照为5 000 lx、NH4+-N∶NO3–-N为0.8∶9.2条件下有利于葡萄试管苗可溶性糖和可溶性蛋白质的积累。
[Objective] The theoretic basis of the management of the grape plantlet in vitro in light intensity and N-fertilization was provided.[Method] The effect of the different light intensities and N-fertilizations（NH4＋-N and NO3–-N） on the growth/development and the physiological characteristic of plantlet in vitro grape variety Red Globe was researched.[Results] The results showed that the content of chlorophyll in the plantlet was increased with the increment of the ratio of NH4＋-N in N-fertilization.The highest content of chlorophyll in the plantlet was reached under the condition of the light intensity of 5 000 lx and the ratio of NH4＋-N to NO3–-N of 5∶ 5.Under the same condition of N-fertilization the content of soluble sugar and protein was improved with the increment of light intensity.When the light intensity was 5 000 lx and the ratio of NH4＋-N to NO3–-N was 0.8∶ 9.2（based on the improved medium B5）,the content of soluble sugar and protein was the highest in all treatments.[Conclusion] The following condition： the light intensity of 5 000 lx and the ratio of NH4＋-N to NO3–-N of 0.8∶ 9.2,was beneficial to the accumulation of the content of soluble sugar and protein.
出处
《安徽农业科学》
CAS
北大核心
2011年第11期6318-6319,6401,共3页
Journal of Anhui Agricultural Sciences
关键词
葡萄试管苗
光照强度
氮素配比
生理特性
Grape plantlet
Light intensity
Ratio of N-fertilization
Physiological characteristics
