摘要
目的:研究泥湖菜乙醇提取物氯仿和乙酸乙酯分离部位化学成分。方法:采用硅胶柱色谱,ODS柱色谱,Sephadex LH-20柱色谱和制备液相色谱等手段进行化学成分分离,运用理化性质和波谱数据(MS,1 H-NMR,13 C-NMR,HMBC等)鉴定化合物结构。结果:从泥湖菜乙醇提取物氯仿和乙酸乙酯分离部位分离得到8个黄酮类化合物,分别鉴定为金合欢素(1),玄参黄酮(2),芹菜素(3),山萘酚(4),山萘酚-3-O-β-D-吡喃葡萄糖苷(5),山萘酚-7-O-β-D-吡喃葡萄糖苷(6),山萘酚-3-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖苷(7),金合欢素-7-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖苷(8)。结论:化合物5为首次从该属植物中分离得到。
Objective：To investigate the chemical constituents from the chloroform and ethyl acetate fractions of ethanol extract from Hemistepta lyrata.Method： The compounds were isolated by silica gel chromatography,ODS chromatography,Sephadex LH-20 chromatography,and preparative HPLC,and their structures were elucidated on the basis of chemical and spectroscopic methods,including MS,1D and 2D NMR spectral techniques.Result：Eight flavonoids were isolated from the chloroform and ethyl acetate fractions of ethanol extract from H.lyrata,and were identified as acacetin（1）,cirsimaritin（2）,apigenin（3）,kaempferol（4）,kaempferol-3-O-β-D-glucopyranoside（5）,kaempferol-3-O-β-D-glucopyranoside（6）,kaempferol-3-O-α-L-rhamnopyranosyl-（1 →6）-β-D-glucopyranoside（7）,and acacetin-7-O-α-L-rhamnopyranosyl-（1 →6）-β-D-glucopyranoside（8）.Conclusion： This is the first report of the isolation of 5 from Hemistepta genus.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第9期107-110,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十一五"科技支撑计划项目(2007BAI38B05)
关键词
泥湖菜
黄酮
化学成分
Hemistepta lyrata
flavonoids
chemical constituent
